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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,612 results for: Nes

Showing: 4481 - 4500 of 24612 results

  1. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid
    #101233
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G582A, V583A, E584A, D585A and N588A
    Promoter
    T7
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101233-plasmid-map-sequence-id-194633

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  4. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163104-plasmid-map-sequence-id-321845

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  10. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid
    #101236
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K918A/V922A/R925A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K918A, V922A, R925A and Q926A
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101236-plasmid-map-sequence-id-197520

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  13. exon L-firefly

    Plasmid
    #81006
    Purpose
    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
    Depositor
    Richard Baines
    Article
    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:
    Insert
    paralytic (para Fly)
    Use
    Tags
    firefly luciferase
    Expression
    Insect
    Mutation
    spanning exon 24 to exon 26, termination codon in…
    Promoter
    P-AC5 (Actin 5C)
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    81006-plasmid-map-sequence-id-158526

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  18. pDRF'- AmTryoshka1;3-LS-F138I

    Plasmid
    #100922
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F138I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100922-plasmid-map-sequence-id-193743

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  19. pDRF'- AmTryoshka1;3-LS-L255I

    Plasmid
    #100964
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    L255I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100964-plasmid-map-sequence-id-193747

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  20. pDRF'- AmTryoshka1;3-GS-F138I

    Plasmid
    #100965
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    F138I
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 4481 - 4500 of 24612 results