We narrowed to 15,734 results for: LEA

1. pBM13g-sd4U

   Plasmid

   #179689

   Purpose

   Bacterial expression of base editor

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd4U] UGI-TALE3-DddA-G1397-N (S1330I + A1341V + N1342S + E1370K + T1380I)
   

   Expression

   Bacterial

   Mutation

   S1330I A1341V N1342S E1370K T1380I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pnYC-NpM-NcPAN2_1-390_Y

   Plasmid

   #148103

   Purpose

   Bacterial Expression of NcPAN2_1-390

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

   Insert

   NcPAN2_1-390
   

   Expression

   Bacterial

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH2028

   Plasmid

   #179771

   Purpose

   Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

   Depositor

   Mei Zhen

   Article

   Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

   Insert

   NLF-1::2XGFP (nlf-1 Nematode)
   

   Tags

   2XGFP

   Expression

   Worm

   Promoter

   nlf-1

   Available Since

   March 14, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pJH2437

   Plasmid

   #179780

   Purpose

   Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

   Depositor

   Mei Zhen

   Article

   Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

   Insert

   NLF-1 (nlf-1 Nematode)
   

   Tags

   no

   Expression

   Worm

   Promoter

   Prgef-1

   Available Since

   March 2, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAG.INBox-mCherry-INBox-eDHFR

   Plasmid

   #176203

   Purpose

   Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   INBox-mCherry-INBox-eDHFR
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-PB1-AG-FRB-TEVuncs-mTagBFP2-HA

   Plasmid

   #178869

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVuncs-mTagBFP-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVuncs(NEYLQFL)-mTagBFP2-HA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-mCherry-Zdk1-Vav2cat

   Plasmid

   #178875

   Purpose

   Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG.mCherry-INBox-eDHFR

   Plasmid

   #176202

   Purpose

   Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   mCherry-INBox-eDHFR
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CAG.mCherry-INBox(AAA)-eDHFR

    Plasmid

    #176207

    Purpose

    INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-INBoxAAA-eDHFR
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TetO::EGFP-hMeikin(8A mut)

    Plasmid

    #174707

    Purpose

    Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH775_pJH_LldR_PlldR-sfGFP.2opal

    Plasmid

    #163734

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding two opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.2TGA
    

    Expression

    Bacterial

    Mutation

    gfp with first two leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-HA-PGRMC1-1-54

    Plasmid

    #173472

    Purpose

    PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 55-194

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-HA-PGRMC1-21-195

    Plasmid

    #173473

    Purpose

    PGRMC1 lacking the N-terminal region upstream of the transmembrane region

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 1-20

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IBMc120

    Plasmid

    #161950

    Purpose

    Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-PECF20-B30-mScarlet-I-B15
    

    Expression

    Bacterial

    Promoter

    PECF20

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IBMc393

    Plasmid

    #161953

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB4A3-T-PT7-B30-mScarlet-I-3T
    

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IBMc330

    Plasmid

    #161952

    Purpose

    Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA171-PSrpR-B30-mScarlet-I-Ter
    

    Expression

    Bacterial

    Promoter

    PSrpR

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IBMc050

    Plasmid

    #161938

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IBMc259

    Plasmid

    #161951

    Purpose

    Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
    

    Expression

    Bacterial

    Promoter

    PTet*

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nup54 no FG-mEGFP494(2)

    Plasmid

    #164271

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge