We narrowed to 14,039 results for: TIM

1. pcDNA3-BAP-Sox2

   Plasmid

   #133281

   Purpose

   Expression of target proteins BAP-Sox2 in mammalian cells

   Depositor

   Arman Kulyyassov

   Article

   Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

   Insert

   Sox2 (SOX2 Human)
   

   Tags

   Biotin Acceptor Peptide contains 7-His-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLK1 gRNA (BRDN0001147678)

   Plasmid

   #76460

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLNCX2-mCherry-CHMP2A-siRNAres

   Plasmid

   #115332

   Purpose

   expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mutated to be resistant to siRNA knockdown

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADCK4 gRNA (BRDN0001145019)

   Plasmid

   #76560

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADCK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADCK4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PTK6 gRNA (BRDN0001149364)

   Plasmid

   #76490

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PTK6

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PTK6 (PTK6 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIP5K1A gRNA (BRDN0001147757)

   Plasmid

   #77963

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1A (PIP5K1A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIP5K1A gRNA (BRDN0001147420)

   Plasmid

   #77964

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1A (PIP5K1A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PIP5K1B gRNA (BRDN0001147624)

   Plasmid

   #77083

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIP5K1B (PIP5K1B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. INCTbiosyn-pAct-AtINT4

   Plasmid

   #127530

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for A. thaliana expression.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCNL-C1_fibrillarin

    Plasmid

    #65709

    Purpose

    Expresses CNL-fibrillarin in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    fibrillarin (FBL Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. FLAG-HA-FbxO10-pcDNA3.1-

    Plasmid

    #52508

    Purpose

    expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO10 (FBXO10 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. non-targeting control gRNA (BRDN0001145269)

    Plasmid

    #80249

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. ROCK2 gRNA (BRDN0001146481)

    Plasmid

    #76350

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK2 (ROCK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sTR056

    Plasmid

    #177369

    Purpose

    Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-NFYC-IDR-mEGFP

    Plasmid

    #185015

    Purpose

    Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    NFYC IDR (Nfyc Mouse)
    

    Expression

    Bacterial

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NUAK1 gRNA (BRDN0001145557)

    Plasmid

    #75575

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7T3-PacI-elfn1_459to1199

    Plasmid

    #87671

    Purpose

    In situ probe for mouse Elfn1

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Elfn1 (Elfn1 Mouse)
    

    Expression

    Bacterial

    Mutation

    partial sequence, (459 to 1199bp off NM_175522.3)

    Promoter

    T7/T3

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. non-targeting control gRNA (BRDN0001148372)

    Plasmid

    #80258

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge