Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,157 results for: pcDNA

Clear keywords
Showing: 11351 - 11400 of 12157 results

  1. pQ25-VC

    Plasmid
    #175062
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ25 (HTT Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175062-plasmid-map-sequence-id-362344

    Enlarge

  3. H3S10ph biosensor

    Plasmid
    #120809
    Purpose
    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    Expression
    Mammalian
    Mutation
    Threonine 3, Threonine 6, and Threonine 11 are mu…
    Promoter
    CMV
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120809-plasmid-map-sequence-id-235281

    Enlarge

  4. pQ39-VC

    Plasmid
    #175061
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ39 (HTT Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175061-plasmid-map-sequence-id-353797

    Enlarge

  7. RANLS-DEVD-BNES

    Plasmid
    #50840
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    50840-plasmid-map-sequence-id-76287

    Enlarge

  9. GANES-DEVD-BNLS

    Plasmid
    #50842
    Purpose
    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddGFP B
    Tags
    NES sequence LALKLAGLDIGS placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50842-plasmid-map-sequence-id-76290

    Enlarge

  10. Epac-S-H81

    Plasmid
    #170339
    Purpose
    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170339-plasmid-map-sequence-id-344640

    Enlarge

  11. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

    Enlarge

  14. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105782-plasmid-map-sequence-id-203371

    Enlarge

  16. Opto-M1R

    Plasmid
    #106069
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-M1R (CHRM1 Bovine, Human)
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106069-plasmid-map-sequence-id-209711

    Enlarge

  18. NESRA-DEVD-BNLS

    Plasmid
    #50849
    Purpose
    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LQKKLEELELDE placed after ddGFP A an…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    50849-plasmid-map-sequence-id-76294

    Enlarge

  22. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Article
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    131124-plasmid-map-sequence-id-257454

    Enlarge

  24. pDisplay-oROS-HT

    Plasmid
    #216416
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    pDisplay-oROS-HT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216416-plasmid-map-sequence-id-430079

    Enlarge

  33. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

    Enlarge

  34. Epac-S-H90

    Plasmid
    #170340
    Purpose
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170340-plasmid-map-sequence-id-335919

    Enlarge

  35. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188257-plasmid-map-sequence-id-374591

    Enlarge

  36. pKK-TEV-mVenus

    Plasmid
    #105791
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mVenus
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105791-plasmid-map-sequence-id-203382

    Enlarge

  37. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

    Enlarge

  38. TKTL1

    Plasmid
    #72419
    Purpose
    Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis
    Depositor
    David Sidransky
    Article
    Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66
    Insert
    TKTL1 (TKTL1 Human)
    Expression
    Mammalian
    Mutation
    may have one or two synonymous substitutions
    Promoter
    CMV
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  41. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

    Enlarge

  44. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid
    #198245
    Purpose
    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted F-actin binding site on a-catenin (849-90…
    Promoter
    CMV
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    198245-plasmid-map-sequence-id-401145

    Enlarge

Showing: 11351 - 11400 of 12157 results