We narrowed to 14,875 results for: pET

1. PB09-TRE-ZNF787_G379A

   Plasmid

   #117320

   Purpose

   Overexpression of ZNF787

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   ZNF787 (ZNF787 Human)
   

   Use

   Transposon-mediated integration

   Tags

   Expression

   Mammalian

   Mutation

   G379A

   Promoter

   Tet-inducible promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3-E-cadherin promoter

   Plasmid

   #61798

   Purpose

   Luciferase reporter containing the mouse E-cadherin promoter

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Lenti-BaeI-Pgk-Cre

   Plasmid

   #173663

   Purpose

   Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   BaeI site
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2-mkgDUX4

   Plasmid

   #21156

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. h-Plekhm1_FLAG-pcDNA 3.1

   Plasmid

   #73593

   Purpose

   Express human Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPRv2 sgBLM10

   Plasmid

   #127643

   Purpose

   Knock-out of human BLM

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 sgRNA (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHS1928

   Plasmid

   #205967

   Purpose

   JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH all proteins, with His-tagged Cas8
   

   Use

   Tags

   14xHis on Cas8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-sgNT.3#3/Cre

   Plasmid

   #173662

   Purpose

   Expresses a NT.3-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   Non-targeting gRNA
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO His6 SUMO PYL1 (36-211)

    Plasmid

    #177843

    Purpose

    Bacterial Expression of PYL1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 1 (PYL1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRF+423Dux4

    Plasmid

    #21625

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    Double homeobox, chr 4 (DUX4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pAAV_-CMV-Pcdh15_mini_V4-CD2

    Plasmid

    #199184

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-CMV-mCherry-hPMCA2x/b

    Plasmid

    #111571

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro-irf3-shrna5

    Plasmid

    #127649

    Purpose

    Knock-down of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 shRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV.U6.gDNMT1.Cas9-T2A-GFP

    Plasmid

    #170362

    Purpose

    Plasmid used for Crispr/cas9 based disruption of human DNMT1.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX5X.1 PINK1 KD

    Plasmid

    #13322

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496; Kinase dead: K219D/D362A/D3…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

17. P119 C-Check

    Plasmid

    #66817

    Purpose

    Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

    Depositor

    Yonglun Luo

    Article

    Zhou et al Cell Mol Life Sci. 2016 Jan 11.

    Insert

    truncated Enhanced Green Fluorescent Protein and asRED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRC/CMV-JAK1K908E-VSV

    Plasmid

    #139357

    Purpose

    Expression of kinase-dead JAK1-K908E protein

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    K908E

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid

    #171137

    Purpose

    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 221 to alanine, tryptophan 453…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide-Puro-IFITM3g1 (BB15)

    Plasmid

    #139460

    Purpose

    Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RepairTemplate_Smc4-mAID-HaloTAG

    Plasmid

    #217658

    Purpose

    Repair template to endogenously tag SMC4 with mAID-Halo Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    SMC4-mAID-HaloTag (SMC4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTAL_RFP

    Plasmid

    #43859

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-sgNT.2#2/Cre

    Plasmid

    #173661

    Purpose

    Expresses a NT.2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTAL_GFP

    Plasmid

    #43858

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GAL4-Creb5 (1- 508)

    Plasmid

    #195030

    Purpose

    GAL4-Creb5 fusion construct (full length, 508aa)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS313_ATP3T911A

    Plasmid

    #120254

    Purpose

    Expression of ATP3-6 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T911 changed to A; Isoleucine 304 changed to Aspa…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRC/CMV-JAK1P1084A-VSV

    Plasmid

    #139358

    Purpose

    Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    P1084A

    Promoter

    CMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hEBI3 pEF6-V5 (MJS_002)

    Plasmid

    #72490

    Purpose

    Expression of human EBI3 in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    EBI3 (EBI3 Human)
    

    Use

    Tags

    His/V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Erk2

    Plasmid

    #40777

    Purpose

    Expression of rat Erk2 in mammalian cells

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

    Insert

    Erk2 (Mapk1 Rattus norvegicus)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

31. hPlekhm1-dsRedM

    Plasmid

    #73592

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

32. 2xNLS-NLP-cMyc LbaCas12a

    Plasmid

    #182123

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc LbaCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pmiRGLO-ADGRL1-201

    Plasmid

    #117336

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR ADGRL1-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

    Plasmid

    #114102

    Purpose

    fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    GLucM23-iChloC-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVenus-CyclinB1(R42A) (1317)

    Plasmid

    #39873

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    CyclinB1(R42A) (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    R42A = destruction box mutations = non-destructib…

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pRF+275Dux4

    Plasmid

    #21293

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX 4 (DUX4 Human, DUX 4)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pRC/CMV-TYK2P1104A-VSV

    Plasmid

    #139350

    Purpose

    Expression of the TYK2-P1104A variant, rs34536443

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-P1104A

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

    Plasmid

    #114101

    Purpose

    fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Zebrafish-gRNA-0004

    Plasmid

    #42244

    Purpose

    gRNA targeted to zebrafish gene fh [site #2]

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-fh site #2 (fh Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only