We narrowed to 18,470 results for: Met

1. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-P3-Alb-mNG

   Plasmid

   #183460

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mNeonGreen

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSynSRE-Mut-T-Luc

   Plasmid

   #60490

   Purpose

   Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988

   Depositor

   Timothy Osborne

   Article

   Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.

   Insert

   HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
   

   Use

   Luciferase

   Tags

   Luciferase reporter

   Expression

   Mammalian

   Mutation

   Four point mutations (G->C or A->C) in the …

   Promoter

   Partial HMG-CoA synthase promoter (-324/-225) fus…

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRuby2-LaminA-C-18

   Plasmid

   #55901

   Purpose

   Localization: Nuclear Envelope, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   LaminA (LMNA Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAG414GPD-dCas9-VPR

   Plasmid

   #63801

   Purpose

   pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

   Plasmid

   #47904

   Purpose

   An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   eNpHR3.0
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-Mm2

   Plasmid

   #197100

   Purpose

   To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   PylRS variant, Pyl tRNA, kanamycin resistance gene
   

   Expression

   Bacterial

   Mutation

   Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

   Plasmid

   #55648

   Purpose

   Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre off/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.2/V5-DEST CDC42EP1 WT

    Plasmid

    #69819

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast FLVCR1b-3X FLAG

    Plasmid

    #218536

    Purpose

    human FLVCR1b with C-terminal 3X_FLAG cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (3X)

    Expression

    Mammalian

    Mutation

    Alternative isoform

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

    Plasmid

    #218534

    Purpose

    human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (3X)

    Expression

    Mammalian

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. M_ST1n_VPR

    Plasmid

    #63799

    Purpose

    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    ST1-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-Chronos-GFP

    Plasmid

    #59170

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FuG-E

    Plasmid

    #67509

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

    Insert

    Fusion Glycoprotein type E
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Promoter

    CAGGS

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC589 - pAAV c-fos Nuc-eYFP

    Plasmid

    #59133

    Purpose

    An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    mFos

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid

    #41733

    Purpose

    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    T7

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATP1A1_plasmid_donor_RD

    Plasmid

    #86551

    Purpose

    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 (ATP1A1 Human)
    

    Use

    CRISPR and TALEN; Co-selection via hdr using ouab…

    Expression

    Mammalian

    Mutation

    Q118R, N129D

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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