We narrowed to 15,987 results for: nes

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pAF020 p3E-2A-mCerulean

Plasmid

#195974

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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460 p3E-2A-mCherrypA

Plasmid

#195970

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

Plasmid

#207859

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PEmax NG

Plasmid

#187894

Purpose

All-in-one prime editor piggyBac transposon with PEmax, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-hMeCP2-GFP-R270X

Plasmid

#48079

Purpose

Mammalian expression of GFP tagged MeCP2-R270X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

expresses AT-Hook R270X

Promoter

CMV

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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MLM3747

Plasmid

#49965

Purpose

Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

3x Flag Tet1CDmut HB-6 (HBB Human)

Use

TALEN

Tags

3x FLAG

Mutation

catalytic domain of TET1 inactive (H1671Y and D16…

Promoter

EF1a

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
MLM3739

Plasmid

#49959

Purpose

Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

3x Flag Tet1CDmut HB-4 (HBB Human)

Use

TALEN

Tags

3x FLAG

Mutation

catalytic domain of TET1 inactive (H1671Y and D16…

Promoter

EF1a

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
612 p3E-2A-mCherryCAAXpA

Plasmid

#195971

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-DmrA

Plasmid

#61680

Purpose

GFP-labeled SAH scaffold with DmrA attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-DmrA

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pPlantPOP_STARBURST

Plasmid

#241284

Purpose

UBQ10 driven expression of STARBURST, a poly-cistronic fungal bioluminescense pathway, in plants

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

STARBURST

Expression

Plant

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGGC025

Plasmid

#48830

Purpose

Provides 3xGFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xGFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1, transient)

Plasmid

#197588

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS vif

Plasmid

#101328

Purpose

Encodes codon optimized NL4-3 vif

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 vif

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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