We narrowed to 4,300 results for: Pet2

1. RanBP2-RB3-4-pET23a

   Plasmid

   #85769

   Purpose

   bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanBP2 fragment (RANBP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   fragment (amino acids 2304-3062)

   Promoter

   T7

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaG-11

   Plasmid

   #172762

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-11
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-SUMO-SpyTag003

   Plasmid

   #216282

   Purpose

   Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SUMO-SpyTag003
   

   Use

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21b-rrt-His8

   Plasmid

   #194088

   Purpose

   Expresses Ec86 reverse transcriptase in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-L713F

   Plasmid

   #174794

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-ETV6-ETS

   Plasmid

   #111180

   Purpose

   Minimal ETS domain of human ETV6 (TEL)

   Depositor

   Gregory Poon

   Article

   Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

   Insert

   ETV6 ETS domain (ETV6 Human, Mouse, Bovine)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET29b-Ava3858-3855

    Plasmid

    #45746

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3855
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21d-colEwt2/Imwt2

    Plasmid

    #107127

    Purpose

    plasmid for expression of WT colicin endonuclease and immunity pair 2

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEwt2/Imwt2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21TtTERT(195-516)

    Plasmid

    #28172

    Depositor

    Kathleen Collins

    Article

    O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

    Insert

    Tetrahymena TERT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a His6Flag2AUF1(p37)

    Plasmid

    #135939

    Purpose

    Expresses AUF1p37 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET21a-mAIM2-His6

    Plasmid

    #51536

    Purpose

    Express His6-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a His6Flag2AUF1(p45)

    Plasmid

    #135942

    Purpose

    Expresses AUF1p45 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-MBP-(CBX1)3-WT

    Plasmid

    #180212

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1 x3 (CBX1 Human)
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HM3C NaVAe1_p pET24b

    Plasmid

    #127854

    Purpose

    Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1p
    

    Use

    Tags

    3C Cleavage site, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-At_PRORP2-His

    Plasmid

    #67870

    Purpose

    bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP2 (PRORP2 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET24a-ncOGT-TPR

    Plasmid

    #190822

    Purpose

    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    C467X. Also codon optimized for expression in E.c…

    Promoter

    T7

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pET28 His10-FcRɣ

    Plasmid

    #113021

    Purpose

    for purification of His10-FcRɣ intracellular domain

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    His10-FcRɣ intracellular domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET24b-6His-fSNAP

    Plasmid

    #106999

    Purpose

    To express 6His-fSNAP protein in E.coli

    Depositor

    Melissa Moore

    Article

    Haraszti et al Methods Mol Biol. 2020;2113:17-22. doi:

    Insert

    fSNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET29b-EPN01*-His

    Plasmid

    #84509

    Purpose

    E.coli version of EPN01*

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN01*-His_pET29b
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pET28b-Ets1-ETS

    Plasmid

    #85735

    Purpose

    Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
    

    Use

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET24a-LaM4-3xTS

    Plasmid

    #162777

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 5787 pET28aSX104B/ pES42

    Plasmid

    #1229

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pET29b-ClbPpep-CHis

    Plasmid

    #48243

    Purpose

    First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP-pep
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    First 375 amino acids of the peptidase, which con…

    Promoter

    T7

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-pro-sumo3

    Plasmid

    #25103

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO3 (SUMO3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Serine 2 to Alanine

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

44. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

47. Brg1 Del1 pET28a

    Plasmid

    #122115

    Purpose

    Expression Brg1 amino acids 1-381 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET28-SUMO-KaiB

    Plasmid

    #68050

    Purpose

    Strain: AM5116, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiB
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a-DDX43-K292A

    Plasmid

    #134575

    Purpose

    Express human DDX43 mutant (K292A, ATPase dead) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-K292A (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (K292A)

    Promoter

    T7

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28-SUMO-KaiA

    Plasmid

    #68049

    Purpose

    Strain: AM5115, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiA
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge