Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,811 results for: Met;

Clear keywords
Showing: 441 - 460 of 9811 results

  1. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

    Enlarge

  7. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

    Enlarge

  13. mAmetrine-LaminB1-10

    Plasmid
    #56547
    Purpose
    Localization: Nuclear Envelope, Excitation: 406, Emission: 526
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Tags
    mAmetrine
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pGBT metaxin 2

    Plasmid
    #12419
    Depositor
    Paul Bornstein
    Insert
    metaxin 2 (Mtx2 Mouse)
    Expression
    Yeast
    Available Since
    Oct. 23, 2006
    Availability
    Academic Institutions and Nonprofits only

  15. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

    Enlarge

  16. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

    Enlarge

  17. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

    Enlarge

  20. CMV-GEX-GECO1

    Plasmid
    #32443
    Purpose
    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEX-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 441 - 460 of 9811 results