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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Publication

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Publication

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Publication

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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LeGO-C2

Plasmid

#27339

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, mCherry

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Publication

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Tags

HA

Expression

Mammalian

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Publication

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Publication

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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LeGO-Cer2

Plasmid

#27338

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Cerulean

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Publication

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pSLCAR-CD19-CD3z

Plasmid

#135993

Purpose

Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-3z-P2A-EGFP (CD247, CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Publication

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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