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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,218 results for: ACE;

Showing: 6901 - 6930 of 20218 results

  1. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  2. MTK0_049

    Plasmid
    #123946
    Purpose
    Encodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hThal5.10-2_hg18 landing pad destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123946-plasmid-map-sequence-id-250330

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  4. R0063 (pLuxR-pR)_AB

    Plasmid
    #66012
    Purpose
    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66012-plasmid-map-sequence-id-114129

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  8. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

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  9. pAV115.loxP

    Plasmid
    #63187
    Purpose
    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63187-plasmid-map-sequence-id-104891

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  10. pAV116.loxP

    Plasmid
    #63188
    Purpose
    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63188-plasmid-map-sequence-id-121508

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  11. pPKm-235

    Plasmid
    #90509
    Purpose
    pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-sHO1, mito-Hy2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    90509-plasmid-map-sequence-id-204693

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  17. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124ā€“3 gene into mCherry. Contains the miR-124-regulated 3ā€²UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124ā€“3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully cā€¦
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59893-plasmid-map-sequence-id-108250

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  18. pZS1[FruR-T]

    Plasmid
    #60752
    Purpose
    Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    FruR-T
    FruR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD andā€¦
    Promoter
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60752-plasmid-map-sequence-id-97733

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  23. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202042
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-1xNLS-[Activ]
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202042-plasmid-map-sequence-id-401647

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  24. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202043
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-[Activ]
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202043-plasmid-map-sequence-id-401604

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  25. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

    Plasmid
    #202044
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Article
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-VP64
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202044-plasmid-map-sequence-id-401598

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  26. MFE23-ZZ

    Plasmid
    #223553
    Purpose
    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.
    Depositor
    Jean Gariepy
    Article
    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6
    Insert
    pET302-MFE23-ZZ
    Use
    Tags
    HA tag and HIS tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223553-plasmid-map-sequence-id-458178

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  30. pM-mKate2

    Plasmid
    #228629
    Purpose
    Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction
    Depositor
    Marc Birtwistle
    Article
    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1
    Insert
    mKate2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    We introduced some silent mutations to avoid esseā€¦
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228629-plasmid-map-sequence-id-457667

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Showing: 6901 - 6930 of 20218 results