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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ220 (AsCpf1)

Plasmid

#86208

Purpose

AsCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1

Use

Gateway compatible cpf1 vectors

Tags

5' and 3' NLS

Expression

Plant

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFP.E227

Plasmid

#138249

Purpose

Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

mCherry
Terminator
mTagBFP
Terminator
gfp
Terminator

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P17_up1691, P20_992, and iP16_3622

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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p5E_unc503

Plasmid

#64020

Purpose

5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)

Use

Gateway Cloning

Promoter

503-bp promoter of zebrafish unc-45b

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-lacZ-stop-L2

Plasmid

#186359

Purpose

Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

lacZ (lacZ Synthetic, E. coli)

Use

Expression of lacz reporter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-MCS

Plasmid

#75174

Purpose

Multiple cloning site for insertion of gene of interest for C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

Multiple Cloning Site

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSR83

Plasmid

#90211

Purpose

Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

OmpR (ompR E. coli)
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-P2A-TurboID-NS

Plasmid

#166568

Purpose

Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

mKate2-P2A-TurboID

Use

Gateway Cloning

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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plk1 T210D

Plasmid

#132964

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Mutation

T210D

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFP.R270

Plasmid

#138219

Purpose

Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFP.R269

Plasmid

#138181

Purpose

Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSW181 - GR-LhG4

Plasmid

#115983

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R268

Plasmid

#138180

Purpose

Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(LNR).gp41-8.(SAV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP-CAAX

Plasmid

#75149

Purpose

Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-iRFP_L5-L4

Plasmid

#113866

Purpose

Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
iRFP713

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR5'-attP (pCM254)

Plasmid

#68311

Purpose

Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

attP

Use

Gateway Cloning

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-CAAX

Plasmid

#75153

Purpose

Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mVenus-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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