We narrowed to 165,271 results for: addgene

1. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-loxp-DsRed-loxp-eGFP

   Plasmid

   #141148

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   loxp-DsRed-loxp-eGFP
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28c-F30-2xdBroccoli-47CAG

   Plasmid

   #237217

   Purpose

   Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

   Depositor

   Mingxu You

   Article

   Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

   Insert

   47 CAG trinucleotide repeats RNA
   

   Expression

   Bacterial

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p65-mNeonGreen

   Plasmid

   #127172

   Purpose

   Fluorescently tagged p65 reporter

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   GFP-RelA (RELA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRia-v2

   Plasmid

   #84832

   Purpose

   CRISPRi/a V2 library parental plasmid

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Inserts

   sgRNA
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-Empty

   Plasmid

   #91980

   Purpose

   Empty backbone

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. gh123

    Plasmid

    #106790

    Purpose

    expression of gRNA targeting ST8SIA3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST8SIA3 (ST8SIA3 Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGDP1 NDM-1

    Plasmid

    #112883

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSpCas9-VPR

    Plasmid

    #205247

    Purpose

    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER20-Src(K295R)

    Plasmid

    #215465

    Purpose

    Dox-inducible lentiviral transfer vector with Src kinase-dead mutant (K295R)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    Src (SRC Chicken)
    

    Use

    Lentiviral

    Mutation

    K295R (Kinase dead)

    Promoter

    mCMV, Doxycycline-inducible

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. nls-transposase-nls-pA in pT3TS-Dest

    Plasmid

    #140873

    Purpose

    Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nls-transposase-nls (Tol2)
    

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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