We narrowed to 13,682 results for: sequence
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Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK88_GFP-Anillin
Plasmid#46354DepositorInsertAnillin (ANLN Human)
UseRetroviralTagsGFPExpressionMammalianMutationa1854 is changed to c to disrupt XhoI site withou…PromoterpBABEAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALPS tat-P2A-rev
Plasmid#101331PurposeLentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)DepositorInsertNL4-3 tat and rev
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB5322
Plasmid#204832PurposeAn integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
TagsN-terminal His tag with thrombin cleavage siteExpressionBacterialAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]
Plasmid#133327PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-41: ATP2A2-mEGFP
Plasmid#114405PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2DepositorInsertATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T2
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
phNFL
Plasmid#132589PurposeExpresses human NFLDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRExpressionWormAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
A1-20B1(P004)
Plasmid#184056PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.DepositorInsertA1-20B1 preamplifier
UseSynthetic BiologyAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Calreticulin-SuperNova-KDEL
Plasmid#131857PurposeExpresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signalDepositorInsertSuperNova
TagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-50: NPM1-mEGFP
Plasmid#109122PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1DepositorInsertNPM1 Homology Arms with linker-mEGFP (NPM1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID-GFP
Plasmid#209389PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneTagsGFPExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS:iGluSnfr.zf1
Plasmid#108356PurposeUAS driven variant of iGluSnFR for zebrafish expressionDepositorInsertUAS:[GlyRa1 secretion sequence]-[iGluSnFR]
UseZebrafish expressionPromoterlacAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-mDux-CA
Plasmid#99284PurposeAll-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)DepositorAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-28:TUBA1B-mTagRFP-T
Plasmid#101785PurposeHomology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
Multi-frame tag
Plasmid#128610PurposeIn the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.DepositorInsertMF tag
ExpressionMammalianAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion Halo-mAID
Plasmid#112852PurposeGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous proteinDepositorInsertHalo-mAID
UseSynthetic BiologyPromoterNoAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-EGFP-ssHRP-TM
Plasmid#31156DepositorInsertssHRP-TM
UseMembrane targeted hrp (mhrp)ExpressionMammalianMutationincludes signal sequence and a transmembrane doma…PromoterCMV promoter including immediate early promoter/e…Available SinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQC-FsCFP-mCherryFP
Plasmid#154836PurposeFrame-shift fluorescent reporter for genomic DNA editingDepositorInserttargeting sequence of VEGF
UseRetroviralExpressionMammalianAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only