We narrowed to 11,748 results for: pcDNA

1. pcDNA3.1-deltaA1-Lock-VWF

   Plasmid

   #124824

   Purpose

   Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF without A1 domain and Lock A2 domain (VWF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 WT MYOC eGLuc2 FLAG

   Plasmid

   #192820

   Purpose

   Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

   Depositor

   John Hulleman

   Article

   Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

   Insert

   myocilin (MYOC Human)
   

   Use

   Tags

   FLAG and enhanced Gaussia luciferase (M43I/M110I)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO HIS DNAJB9

   Plasmid

   #19559

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-LplA(AAG)

   Plasmid

   #61307

   Purpose

   Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Use

   Tags

   6xHis, Flag, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-hygro-eGFP-HEC1

   Plasmid

   #114027

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-human C-MYC-V394D

   Plasmid

   #74162

   Purpose

   Mammalian expression of human cMyc VD mutant

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V394D

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

   Plasmid

   #112840

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Full length Grb2

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194684

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-sACE2v2(732)-IgG1

    Plasmid

    #154105

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-optoB2-LYY-YFP

    Plasmid

    #75271

    Purpose

    G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    G-protein independent version of optoB2
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    point mutations at L72F,Y136G,Y224A

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-S586A-VLCAD-FLAG

    Plasmid

    #55824

    Purpose

    Expresses S586A VLCAD mutant in mammalian cells

    Depositor

    Yoshimi Homma

    Article

    Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

    Insert

    Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    changed Serine 586 toAlanine

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid

    #136454

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3 Flag MKK7a1 (K76A)

    Plasmid

    #14541

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(K76A) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Lys-76Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-kappa-HA-dL5-BKa

    Plasmid

    #73212

    Purpose

    Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

    Insert

    BKalpha (Kcnma1 Mouse)
    

    Use

    Tags

    dL5**

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

    Plasmid

    #112829

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-HtrA2-EGFP S306A

    Plasmid

    #14122

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S306A

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FLAG-DDB1 (1140H)

    Plasmid

    #174849

    Purpose

    Expression of DDB1

    Depositor

    Helen Morrison

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge