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We narrowed to 52,941 results for: plasmid

Showing: 11701 - 11750 of 52941 results

  1. pAS2-chCALEBcyt (aa 522-551)

    Plasmid
    #89407
    Purpose
    Two-hybrid system
    Depositor
    Fritz Rathjen
    Insert
    CSPG5 (CSPG5 Chicken)
    Use
    Tags
    GAL4 DBD
    Expression
    Yeast
    Mutation
    aa 522-551
    Promoter
    ADH1
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89407-plasmid-map-sequence-id-177477

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  3. FTCAR1:pFlagCMV-chCAR-SIV

    Plasmid
    #87238
    Purpose
    Mammalian expression of chicken CAR, SIV isoform
    Depositor
    Fritz Rathjen
    Insert
    CXADR (CXADR Chicken)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87238-plasmid-map-sequence-id-172977

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  4. 5J3U (TgPKA, 583.m05422)

    Plasmid
    #79525
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    583.m05422
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79525-plasmid-map-sequence-id-150111

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  5. 5FEA (LdBRD19, LdBPK_363520.1)

    Plasmid
    #79520
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363520.1
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79520-plasmid-map-sequence-id-150104

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  6. 5CZG (Tb427.10.7420)

    Plasmid
    #79517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427.10.7420
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79517-plasmid-map-sequence-id-150098

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  7. 4Z31

    Plasmid
    #69958
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 4Z0O

    Plasmid
    #69954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 4YVD

    Plasmid
    #69960
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLRG1:JMC102-C09:C233452 (PLRG1 Human)
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 4QQM

    Plasmid
    #61950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. 4MYI

    Plasmid
    #60103
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Expression
    Mutation
    See comments
    Promoter
    polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pUASp YFP Mtl DN

    Plasmid
    #52252
    Purpose
    Drosophila expression of YFP-Mtl dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Mtl (Mtl Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T20N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pUASp YFP Mtl CA

    Plasmid
    #52253
    Purpose
    Drosophila expression of YFP-Mtl constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Mtl (Mtl Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q64L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pUASp YFP Rac2 WT

    Plasmid
    #52236
    Purpose
    Drosophila expression of YFP-Rac2
    Depositor
    Matthew Scott
    Insert
    Rac2 (Rac2 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. LZRSMS-GFP-hFJX1-Myc

    Plasmid
    #46932
    Purpose
    retroviral expression of FJX1-myc and GFP
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Retroviral
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. LZRSMS-GFP-hFJX1-Flag

    Plasmid
    #46929
    Purpose
    retroviral expression of FJX1-FLAG and GFP
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. mouse KGFR NEG D

    Plasmid
    #19272
    Depositor
    Philip Leder
    Insert
    mKGFR (negative dominant) (Fgfr2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 19, 2008
    Availability
    Academic Institutions and Nonprofits only

  18. pET28aHisAviCD72aCTLD

    Plasmid
    #129067
    Purpose
    generation of biotinylated mouse CD72a CTLD protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    CD72a (Cd72 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    129067-plasmid-map-sequence-id-252110

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  19. pET28aHisAviCD72cCTLD

    Plasmid
    #129068
    Purpose
    generation of biotinylated mouse CD72c CTLD protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    CD72c (Cd72 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    129068-plasmid-map-sequence-id-252114

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  20. pMXCD72a-ires-YFP

    Plasmid
    #129070
    Purpose
    expression of mouse CD72a in animal cells
    Depositor
    Takeshi Tsubata
    Insert
    CD72a (Cd72 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    129070-plasmid-map-sequence-id-252181

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  21. pMXCD72c-ires-YFP

    Plasmid
    #129072
    Purpose
    expression of mouse CD72c in animal cells
    Depositor
    Takeshi Tsubata
    Insert
    CD72c (Cd72 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    129072-plasmid-map-sequence-id-252418

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  22. pMXs-CD72a-IRES-hCD8

    Plasmid
    #129074
    Purpose
    expression of mouse CD72a in animal cells
    Depositor
    Takeshi Tsubata
    Insert
    CD72a (Cd72 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    129074-plasmid-map-sequence-id-252178

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  23. StarPort02

    Plasmid
    #164504
    Purpose
    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
    Depositor
    Bo Huang
    Insert
    V5-SNAPtag-PDGFR_TM-T2A-Blast
    Use
    To generate attp-ga acceptor cell lines for bxbi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human RPL30
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164504-plasmid-map-sequence-id-324721

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  24. pAN289

    Plasmid
    #164524
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    eGFP-Tev-S
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164524-plasmid-map-sequence-id-328003

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  25. IDG_FAM26D_OE_1

    Plasmid
    #161670
    Purpose
    Overexpresses FAM26D
    Depositor
    Mike McManus
    Insert
    FAM26D (FAM26D Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161670-plasmid-map-sequence-id-319662

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  26. IDG_KCNA7_OE_1

    Plasmid
    #161680
    Purpose
    Overexpresses KCNA7
    Depositor
    Mike McManus
    Insert
    KCNA7 (KCNA7 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161680-plasmid-map-sequence-id-334567

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  27. IDG_KCNIP1_OE_1

    Plasmid
    #161690
    Purpose
    Overexpresses KCNIP1
    Depositor
    Mike McManus
    Insert
    KCNIP1 (KCNIP1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161690-plasmid-map-sequence-id-319712

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  28. IDG_KCNMB4_OE_1

    Plasmid
    #161692
    Purpose
    Overexpresses KCNMB4
    Depositor
    Mike McManus
    Insert
    KCNMB4 (KCNMB4 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161692-plasmid-map-sequence-id-319367

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  29. IDG_LRRC55_OE_1

    Plasmid
    #161694
    Purpose
    Overexpresses LRRC55
    Depositor
    Mike McManus
    Insert
    LRRC55 (LRRC55 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161694-plasmid-map-sequence-id-319728

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  30. IDG_ASIC4_OE_1

    Plasmid
    #161636
    Purpose
    Overexpresses ASIC4
    Depositor
    Mike McManus
    Insert
    ASIC4 (ASIC4 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161636-plasmid-map-sequence-id-319566

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  31. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  32. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

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  33. pCiGSΔΨ

    Plasmid
    #108312
    Purpose
    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    David Russell
    Article
    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.
    Insert
    gag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    pCiGSΔΨ is a newer version of the pCiGS Gag expre…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108312-plasmid-map-sequence-id-211440

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  34. pTSin EGFP

    Plasmid
    #127695
    Purpose
    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127695-plasmid-map-sequence-id-398129

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  36. pSRE SSG

    Plasmid
    #127692
    Purpose
    Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    Sindbis Structural Genome
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127692-plasmid-map-sequence-id-392665

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  38. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mammalian Pol I
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  39. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  42. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  44. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

    Enlarge

  45. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  46. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

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  50. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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Showing: 11701 - 11750 of 52941 results