12,913 results

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

Plasmid

#51247

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-LC-Myosin-C-10

Plasmid

#54145

Purpose

Localization: Light Chain Myosin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LC-Myosin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-MAP4-C-10

Plasmid

#57399

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

MAP4

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits
1kb Zfp521 promoter reporter

Plasmid

#104188

Purpose

luciferase reporter to assay Zfp521 promoter activation

Depositor

Kathryn Hentges

Article

Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

Insert

Zfp521 promoter - 1 Kb (Zfp521 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

none

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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FAM19A1-V5

Plasmid

#184337

Purpose

Expression of V5-tagged mouse FAM19A1

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

FAM19A1 (Tafa1 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV_PIG_3XHA-Ago1

Plasmid

#170916

Purpose

Expresses 3X-HA-AGO1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

Ago1 (Ago1 Mouse)

Tags

3X-HA

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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p2XHA-Borcs7

Plasmid

#118746

Purpose

Expresses 2XHA tagged Borcs7

Depositor

Beverly Koller

Insert

Borcs7 cDNA (2010012O05Rik Mouse)

Tags

2XHA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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MTK2_024

Plasmid

#123716

Purpose

Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1c

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-M71-IRES-tauRFP2ACNf

Plasmid

#15510

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES tauRFP-RFP ACN

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 216C

Plasmid

#17523

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-215

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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DT050A

Plasmid

#159411

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEos4b-MKS1-N-18

Plasmid

#62776

Purpose

Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

MKS1

Tags

mEos4b

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pISH-Lhx2

Plasmid

#74337

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

LIM homeobox protein 2 (Lhx2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pMCh2007_pLenti_Syn_mChe-cdc42E7

Plasmid

#118620

Purpose

expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed cagtctg to ggcataa (667 - 673 nt in 3…

Promoter

synapsin I (rat)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNoxa1

Plasmid

#58339

Purpose

expresses mouse Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (Noxa1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-shMdk-334

Plasmid

#99025

Purpose

Expresses shRNA targeting mouse Mdk (midkine)

Depositor

Amy Lasek

Article

Chen et al Genes Brain Behav. 2017 Apr 11. doi: 10.

Insert

Mdk shRNA (Mdk Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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mILK-L207W-EFGP

Plasmid

#176900

Purpose

mouse ILK with L207W point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Leucine 207 to Tryptophane

Promoter

CNV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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mTFP1-LC-Myosin-N-7

Plasmid

#55494

Purpose

Localization: Light Chain Myosin, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LC-Myosin

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgDnmt3a#1/Cre

Plasmid

#173587

Purpose

Expresses a Dnmt3a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Dnmt3a (Dnmt3a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FAK

Plasmid

#186141

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mSTIM1_1-310-CFP

Plasmid

#69551

Purpose

Expresses a STIM1_1-310 fragment mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

mSTIM1_1-310

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only
MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pGEX2T-p18Ink4c

Plasmid

#52218

Purpose

bacterial expression of p18Ink4c

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4c (Cdkn2c Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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12,913 results