We narrowed to 20,219 results for: ACE;

1. pBADHISTEV_PfeEH258A

   Plasmid

   #129122

   Purpose

   expresses H258A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBADHISTEV_PfeES157A

   Plasmid

   #129121

   Purpose

   expresses S157A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

   Plasmid

   #127325

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

   Plasmid

   #127324

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

   Plasmid

   #127327

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

   Plasmid

   #127326

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-3NLS-Sox2TALE-GCN4

   Plasmid

   #120548

   Purpose

   Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a-3NLS-NGN2TALE-GCN4

   Plasmid

   #120547

   Purpose

   Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCRII apoA-Ib

   Plasmid

   #121896

   Purpose

   Plasmid for making zebrafish apoA-Ib in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA1b (apoa1b Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCRII apoA-IVa

    Plasmid

    #121902

    Purpose

    Plasmid for making zebrafish apoA-IVa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoA4a (apoa4a Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a-3NLS-Klf4 TALE-GCN4

    Plasmid

    #120546

    Purpose

    Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TIAM2_PDZ_1

    Plasmid

    #103952

    Purpose

    Protein expression and purification of TIAM2 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TIAM2 (TIAM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPKm- 227

    Plasmid

    #90498

    Purpose

    pcDNA3 - CMVmin - VPR - PIF3, expresses VPR transactivator domain and PIF3, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    VP64-PIF3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPKm-113

    Plasmid

    #90495

    Purpose

    pcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    MTAD-PIF6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid

    #70195

    Purpose

    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    tetR-deGFP fusion
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. R0063 (pLuxR-pR)_EB

    Plasmid

    #66013

    Purpose

    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Controllable promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R0063 (pLuxR-pR)_FB

    Plasmid

    #66014

    Purpose

    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Controllable promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R0063 (pLuxR-pR)_GB

    Plasmid

    #66015

    Purpose

    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Controllable promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available Since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZM3[I:FruR-L,I:RbsR-L]

    Plasmid

    #60777

    Purpose

    Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    FruR-L
    RbsR-L
    

    Use

    Tags

    Expression

    Mutation

    LacI/GalR repressor chimera. LacI DBD with FruR L…

    Promoter

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP C1 SADBs shRNA Resistant

    Plasmid

    #67155

    Purpose

    pEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNA

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    nucleotide 294 C-T, nucleotide 297 C-G

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pSG5 N-HA SADBs shRNA resistant

    Plasmid

    #67157

    Purpose

    N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

    Promoter

    SV40 promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pTER SADB shRNA Human

    Plasmid

    #67151

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    BRSK1 (BRSK1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pZS1[TreR-L,LacI-L]

    Plasmid

    #60760

    Purpose

    Contains PI driving expression TreR-L, and PI driving expression of LacI-L

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    TreR-L
    Dimeric LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with TreR L…

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZS1[RbsR-L,LacI-L]

    Plasmid

    #60757

    Purpose

    Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    RbsR-L
    Dimeric LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with RbsR L…

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgFFL_7x_Bulge

    Plasmid

    #59894

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has seven bulge targets (fully comple…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLEX_970_puro_DEST_V5_hcRED

    Plasmid

    #45286

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    hcRed
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pWzl_neo_DEST_flag_hcRED

    Plasmid

    #45458

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    hcRED
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

30. 35S:5mARF17 pLBR19

    Plasmid

    #12073

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    5mARF17 (ARF17 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    miR160-resistant version of ARF17 has five silent…

    Promoter

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only