We narrowed to 7,325 results for: 11
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Plasmid#194998PurposeProduction of ACE2 extracellular domainDepositorAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRExpressionMammalianPromoterPgkAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha11-RLuc8
Plasmid#140983PurposeEncodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha11-RLuc8 (GNA11 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-GFP
Plasmid#115495PurposeLentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)DepositorInserteGFP
UseLentiviralAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyExpressionInsect and MammalianPromoterEF1aAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-V5 IRES-puro
Plasmid#107058PurposeExpression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationwild-typePromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-PH-PLCD1
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
TagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs T3950D
Plasmid#83320PurposeDNA-PKcs T3950ADepositorInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutationActivation loop phosphorylation site 3950 substit…Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-sfGFP
Plasmid#107057PurposeExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationK785RPromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianPromoterEF-1aAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW411d
Plasmid#246585Purposedifferent combination of A. thaliana and N. tabacum Rubisco assembly factorsDepositorInsertExpressionBacterialAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVC-FUS-R521C
Plasmid#175067Purposeexpresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
TagsHA and mVenus + mCherryExpressionMammalianMutationchanged Arginin 521 to Cystein; mVenus: C-Termina…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mGSDME
Plasmid#154877PurposeExpresses mGSDME in mammalian cellsDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETcon_SARS-CoV-2_RBD
Plasmid#166782PurposeYeast surface display vector, expressing the SARS-2 RBD, 201aa constructDepositorInsertSpike receptor binding domain (RBD) from SARS-CoV-2 (S )
ExpressionYeastAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRExpressionMammalianMutationN57GAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-p53(K164R)
Plasmid#72555PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.DepositorInsertp53 (TP53 Human)
Tags5XHREExpressionMammalianMutationK164RPromoterE1B minimal promoterAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorInserthyA3A-BE4max
UseCRISPRExpressionMammalianAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Tbx20
Plasmid#72697PurposeDoxycyline regulated expression of Tbx20 in mammalian cellsDepositorAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT_D355N
Plasmid#46988PurposeInducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationMutation aspartic acid 355 to asparagine (D355N);…PromotertacAvailable SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Baf60c
Plasmid#72691PurposeDoxycyline regulated expression of Baf60c in mammalian cellsDepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
hyBE4max
Plasmid#157942PurposeMammalian expression of hyBE4maxDepositorInserthyBE4max
UseCRISPRExpressionMammalianAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-V5 IRES-puro
Plasmid#107059PurposeExpression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationK785RPromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-p53(K120R)
Plasmid#72554PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.DepositorInsertp53 (TP53 Human)
Tags5XHREExpressionMammalianMutationK120RPromoterE1B minimal promoterAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-myr-AKT-HA
Plasmid#31790DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA1
Plasmid#223519Purposeknocking out hGSDME in human cellsDepositorAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only