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61,467 results

Showing: 11851 - 11900 of 61467 results

  4. pD649-HAsp-FCGR2A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156590
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCGR2A (FCGR2A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156590-plasmid-map-sequence-id-498568

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  8. SYNJ1_BirA_Nterm

    Plasmid
    #221523
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Tags
    BirA
    Expression
    Mammalian
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221523-plasmid-map-sequence-id-439225

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  13. HiBiT-SRPK1 Fusion Vector

    Plasmid
    #236908
    Purpose
    Express HiBiT-SRPK1 in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    SRPK1 (SRPK1 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236908-plasmid-map-sequence-id-473390

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  20. MLNR-DuET

    Plasmid
    #213339
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MLNR (MLNR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213339-plasmid-map-sequence-id-430913

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  30. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  32. mEmerald-Claudin7-C-12

    Plasmid
    #54041
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Claudin7 (CLDN7 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. TFORF2200

    Plasmid
    #143352
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU4F1 (POU4F1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143352-plasmid-map-sequence-id-400182

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  41. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Available Since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. CXCR6-DuET

    Plasmid
    #213223
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR6 (CXCR6 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213223-plasmid-map-sequence-id-430785

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  50. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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Showing: 11851 - 11900 of 61467 results