We narrowed to 7,181 results for: RAP

1. VfAU1-LOV_P2A_casp9

   Plasmid

   #131224

   Purpose

   Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   VfAU1-LOV-HA_P2A_myc-casp9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_355

   Plasmid

   #187159

   Purpose

   Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

   Depositor

   John Doench, David Root

   Article

   Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-N-P199L

   Plasmid

   #177949

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P199L) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P199L

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH_Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65267

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-Flag-hOct4

   Plasmid

   #20072

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hOct4 (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn-Ace-mNeon2

   Plasmid

   #195518

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   Synapsin

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE ENTR CMV Renilla Luciferase L5-L2

   Plasmid

   #62186

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   Renilla luciferase
   

   Use

   Luciferase; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. C1-mCitrine-FKBP-EZRabd(t567D)

   Plasmid

   #155227

   Purpose

   Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Changed Thr 567 to Asp in original Ezrin

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mKate2-P2A-APEX2-TAPP1-PH

   Plasmid

   #67662

   Purpose

   Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-TAPP1-PH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXJ-Myc-KLF2

    Plasmid

    #232654

    Purpose

    transient expresses KLF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

    Plasmid

    #199354

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tom20-CR

    Plasmid

    #171461

    Purpose

    Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-EGFP-RIP3

    Plasmid

    #75165

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIP3

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIP3 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW212

    Plasmid

    #172196

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK294 (mAID-mCherry2-Bsr)

    Plasmid

    #72832

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV2_CAG_oROS-G

    Plasmid

    #216115

    Purpose

    Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Opto-casp9_AtCRY2-PHR-casp9

    Plasmid

    #131221

    Purpose

    Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    Opto-casp9 (AtCRY2-PHR-casp9)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK291 (mAID-mClover-Bsr)

    Plasmid

    #72829

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ii-Str_TNF-SBP-EGFP

    Plasmid

    #65280

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge