59,428 results

1. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-mitoDsRed

   Plasmid

   #44386

   Purpose

   3rd generation lentiviral vector

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoDsRed
   

   Use

   Lentiviral

   Tags

   61 aa targeting sequence of the P1 isoform F1F0-A…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-FURIN

   Plasmid

   #158442

   Purpose

   Gateway Cloning compatible entry vector for the human FURIN gene.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   FURIN (FURIN Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJC5-Tmem192-3xHA

   Plasmid

   #102930

   Purpose

   Lentiviral expression of a lysosomal tag (Tmem192-HA)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-puro-hTERT

   Plasmid

   #1771

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tau (P301L)-VC

   Plasmid

   #87633

   Purpose

   expresses hTau fused to the C-terminal part of Venus

   Depositor

   Tiago Outeiro

   Insert

   MAPT (MAPT Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Mutation P301L

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

   Plasmid

   #122147

   Purpose

   Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-PPARA

    Plasmid

    #169019

    Purpose

    Expression of PPARA driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human ppara (PPARA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSI-Check2-hRluc-NFkB-firefly

    Plasmid

    #106979

    Purpose

    Luciferase reporter with internal control for NFkB signal.

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    NFKB recognition sites (NFKB1 Human)
    

    Use

    Luciferase

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. F-MED26

    Plasmid

    #15367

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED26 (MED26 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX-hN1ICD

    Plasmid

    #91897

    Purpose

    Doxycycline inducible expression of human Notch1 ICD

    Depositor

    Julien Sage

    Insert

    Notch1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    codons 1770 to 2555 of human NOTCH1

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-OTUB1 WT-pcDNA3.1

    Plasmid

    #118209

    Purpose

    Expresses FLAG-OTUB1 WT in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Has Service

    AAV1, AAV2, and AAV8

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MS2-HA-HsSmg5_T

    Plasmid

    #147552

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5 (SMG5 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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