58,350 results

1. pHR-FUSN-mCh-Cry2olig

   Plasmid

   #101224

   Purpose

   FUS(1-214) fused to mCh-Cry2olig

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   FUS(aa1-214) (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-Cry2olig

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBABE-puro-hTERT

   Plasmid

   #1771

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-CMV-KIF2C

   Plasmid

   #205994

   Purpose

   Over-expression of GFP-KIF2c

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   KIF2c (KIF2C Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-Frankenbody-FLAG-Halo

   Plasmid

   #175296

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with HaloTag
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK444 (EF1-OsTIR1(F74G) Puro)

   Plasmid

   #214374

   Purpose

   EF1-OsTIR1(F74G) (PiggyBac)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   EF1-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

   Plasmid

   #138147

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-Puro-TDP-43-WT

   Plasmid

   #133753

   Purpose

   Wild type human TDP-43 in pLVX-Puro lentiviral vector

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-TFEB

   Plasmid

   #38119

   Purpose

   Mammalian expression of Transcription factor EB fused to EGFP

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-GRAB-rDA3h

    Plasmid

    #208703

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-alphasynuclein-WT

    Plasmid

    #40822

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1683

    Plasmid

    #142826

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TWIST1 (TWIST1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-3FLAG-EEA1

    Plasmid

    #176491

    Purpose

    For lentiviral transduction of 3FLAG-EEA1

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge