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Showing: 1151 - 1200 of 17298 results

  2. pENTR4 HopU1

    Plasmid
    #228395
    Purpose
    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Article
    Simranjit et al bioRxiv 2023.09.25.558804
    Insert
    HopU1
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Alanine inserted after start Methionine due to Nc…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    228395-plasmid-map-sequence-id-462965

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  4. pCF702

    Plasmid
    #209650
    Purpose
    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
    Depositor
    Bastian Molitor
    Article
    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7
    Inserts
    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    209650-plasmid-map-sequence-id-416212

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  11. pENTR4 genomic A. thaliana PLDGAMMA3

    Plasmid
    #209188
    Purpose
    pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Article
    Simranjit et al bioRxiv 2023.09.25.558804
    Insert
    AT4G11840 (PLDGAMMA3 Mustard Weed)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    209188-plasmid-map-sequence-id-415375

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  25. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid
    #168047
    Purpose
    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
    Depositor
    Michael Nash
    Article
    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, ddFLN4, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168047-plasmid-map-sequence-id-334769

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  26. PCYOX1L-bio-His

    Plasmid
    #53410
    Purpose
    Expresses full-length Prenylcysteine oxidase-like precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PCYOX1L (PCYOX1L Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53410-plasmid-map-sequence-id-83193

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  27. PRR4-bio-His

    Plasmid
    #52075
    Purpose
    Expresses full-length Proline-rich protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PRR4 (PRR4 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52075-plasmid-map-sequence-id-80300

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  28. TM9-bio-His

    Plasmid
    #51831
    Purpose
    Expresses full-length Transmembrane 9 superfamily member 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TM9 (TM9SF4 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51831-plasmid-map-sequence-id-79668

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  29. G6B-bio-His

    Plasmid
    #51644
    Purpose
    Expresses full-length Protein G6b precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    G6B (MPIG6B Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51644-plasmid-map-sequence-id-78966

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  30. pCR4-*mRFP

    Plasmid
    #47118
    Purpose
    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
    Depositor
    Stephen Ekker
    Article
    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub
    Insert
    *mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    No start codon
    Promoter
    Lac
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    47118-plasmid-map-sequence-id-67205

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  31. mutpRL-TK 6xUTR

    Plasmid
    #40761
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    6 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. mutpRL-TK 2xUTR

    Plasmid
    #40757
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    2 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. mutpRL-TK 4xUTR

    Plasmid
    #40759
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    4 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. mutpRL-TK 5xUTR

    Plasmid
    #40760
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    5 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. mutpRL-TK 1xUTR

    Plasmid
    #40756
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. PTPRC-bio-His

    Plasmid
    #51813
    Purpose
    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PTPRC (PTPRC Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51813-plasmid-map-sequence-id-79539

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  40. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127370-plasmid-map-sequence-id-246351

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  43. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  45. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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  49. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Article
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

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Showing: 1151 - 1200 of 17298 results