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Showing: 1151 - 1200 of 1457 results

  6. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  8. pET-APOBEC1-YTH

    Plasmid
    #200158
    Purpose
    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTH
    Use
    Tags
    6xHis, HA, and Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200158-plasmid-map-sequence-id-396323

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  12. pHIT179

    Plasmid
    #204510
    Purpose
    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204510-plasmid-map-sequence-id-403822

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  24. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  31. pHIT203

    Plasmid
    #204512
    Purpose
    Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C04F12.1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204512-plasmid-map-sequence-id-403815

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Showing: 1151 - 1200 of 1457 results