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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 24,010 results for: Nes

Showing: 1151 - 1200 of 24010 results

  8. pQP-2460

    Plasmid
    #138973
    Purpose
    Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
    Depositor
    Vladislav Verkhusha
    Article
    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:
    Insert
    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138973-plasmid-map-sequence-id-291734

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  21. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Roland Eils, Barbara Di Ventura
    Article
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

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  27. pQP-2376

    Plasmid
    #138974
    Purpose
    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
    Depositor
    Vladislav Verkhusha
    Article
    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:
    Insert
    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138974-plasmid-map-sequence-id-291767

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  28. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  31. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid
    #164193
    Purpose
    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)
    Depositor
    Thomas Oertner
    Article
    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820
    Inserts
    CamPARI2
    Human α Tubulin
    Use
    AAV
    Tags
    6 His tag and NES
    Expression
    Mammalian
    Mutation
    F391W-L398V
    Promoter
    Syn
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    164193-plasmid-map-sequence-id-325364

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  34. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  41. Bxb1_AJMA

    Plasmid
    #183760
    Purpose
    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003
    Inserts
    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    Use
    Tags
    Lck and NES
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183760-plasmid-map-sequence-id-460683

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  42. pSK267

    Plasmid
    #129272
    Purpose
    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Insert
    NLS-VP16-ZF 43-8-NES
    Use
    Tags
    6xHA
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129272-plasmid-map-sequence-id-255863

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  43. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
    Use
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

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  44. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  46. pAOJ15

    Plasmid
    #91567
    Purpose
    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline promoter
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    91567-plasmid-map-sequence-id-181626

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  47. pDGM6

    Plasmid
    #110660
    Purpose
    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
    Depositor
    David Russell
    Article
    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.
    Insert
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110660-plasmid-map-sequence-id-337382

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Showing: 1151 - 1200 of 24010 results