-
Plasmid#74609PurposeExpresses Hook domain and first coiled coil of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28 mSmo-Cterm
Plasmid#58703Purposeused for anti-mouse Smoothened antibody production (for affinity purification)DepositorInsertSmo (Smo Mouse)
UseTags6X-HISExpressionBacterialMutationcontains C terminus (aa 543-793)PromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsHis6x and mGFPmut3ExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorInsertfull length me31B (me31B Fly)
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3857
Plasmid#45744DepositorInsertAva3858-3857
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S400D
Plasmid#16156DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS400DPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 G399S
Plasmid#16158DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationG399SPromoterT7Available sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-deltaPYD
Plasmid#51522PurposeExpress His6-tagged HIN domain of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
UseTagsHis6 and T7ExpressionBacterialMutationdeletion of pyrin domainPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S142A
Plasmid#16153DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS142APromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-C63A
Plasmid#90302Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationC63APromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p40)
Plasmid#135940PurposeExpresses AUF1p40 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP3-His
Plasmid#67871Purposebacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tagDepositorInsertPRORP3 (PRORP3 Mustard Weed)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
UseTagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S142D
Plasmid#16154DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS142DPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Val
Plasmid#50724Purposeoverexpression of the human TPI1 Ile170Val allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ValinePromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b-CeNLuc-6xHis
Plasmid#135933PurposeExpresses the CeNLuc LumiFluor in bacterial cells.DepositorInsertCeNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
6his R.3-AR GUSpET28a+/pLysS
Plasmid#61157PurposeN-terminal His tagged round 3 aldehyde-resistant GUS expression vectorDepositorInsertGUSAR
UseTags6x HisExpressionBacterialMutationN66D, D151N, A219V, I396T, T480A, Q498R, D508E an…PromoterT7Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1(deltaIB)
Plasmid#73041PurposeBacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1(deltaIB) (Dnm1l Mouse)
UseTags6xHis-tag and PreScission Protease siteExpressionBacterialMutationDeleted amino acids 514-602PromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVSp1_p pET24b
Plasmid#127852PurposeBacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVSp1
UseTags3C Cleavage site, His Tag, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S400A
Plasmid#16155DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS400APromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160
Plasmid#74607PurposeExpresses Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26-CNTN149S/F366A
Plasmid#100165Purposerecombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target proteinDepositorInsertConcentrative nucleoside transporter
UseTagsMBP-HisExpressionBacterialMutationN149S, F366APromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-164
Plasmid#100724PurposeFull-length human TIG3 in E.coli expression vectorDepositorInsertRARRES3 (PLAAT4 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Dog 1-77)
Plasmid#62060PurposeExpression of Dog p53 (residues 1-77) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-77PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-132
Plasmid#100720PurposeExpression of N-terminal region of human TIG3 in E.coliDepositorInsertRARRES3 (PLAAT4 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01(∆L1+∆L2)-His
Plasmid#84511PurposeE.coli version of EPN01(∆L1+∆L2)DepositorInsertEPN01(∆L1+∆L2)-His
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P27A)
Plasmid#62068PurposeExpression of human p53 (1-73) (P27A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P27APromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Axin GID 1-2/pET29b
Plasmid#16307DepositorInsertAxin GID 1-2 (axin1.L Frog)
UseTagsHisExpressionBacterialMutationAmino acids 320-429PromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET23b-proMMP-7-E215Q
Plasmid#29642DepositorInsertproMMP-7 (MMP7 Human)
UseTagsExpressionBacterialMutationE215Q Catalytically InactivePromoterAvailable sinceApril 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-hFX(I154F)-HA
Plasmid#14992DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag-CNA35
Plasmid#215457PurposeEncodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.DepositorInsertCNA35
UseTagsHaloTagExpressionBacterialMutationIncludes stop codonPromoterAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-RANGAP1-CTD
Plasmid#228947Purposeexpress the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimizedDepositorInsertRANGAP1-CTD (RANGAP1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationcontains aa 418-587 onlyPromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-msfGFP-NPM1
Plasmid#237670PurposeFor expression of msfGFP-NPM1 in EcoliDepositorInsertNPM1 (NPM1 Human)
UseTagsmsfGFPExpressionBacterialMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-PKD
Plasmid#237521PurposeRecombinant protein expressionDepositorInsertRwt4_pseudokinase
UseTagsExpressionBacterialMutationPseudokinase region of RWT4PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-RWT4Ca
Plasmid#237518PurposeRecombinant protein expressionDepositorInsertRwt4Ca
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-RWT4Ca_noKDup
Plasmid#237535PurposeRecombinant protein expressionDepositorInsertRwt4Ca_noKDup
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Hf-RfxCas13d-His
Plasmid#234480PurposePlasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)DepositorInsertRfxCas13d
UseCRISPRTags6xHisExpressionBacterialMutationChanged four alanine to valine in positions 134, …PromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-DiCas7-11-His
Plasmid#234482PurposePlasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)DepositorInsertDiCas7-11
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEt28a-SilkTag-CBM-H6
Plasmid#230903PurposeBacterial expression of SilkTagDepositorInsertSilkTag-CBM-H6
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEt28a-H6-SUMO-SilkCatcher
Plasmid#230902PurposeBacterial expression of SilkCatcherDepositorInsertH6-SUMO-SilkCatcher
UseTagsHisTag, thrombin, SUMOExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ImpBeta1-mEosEM
Plasmid#218933PurposeExpress ImpBeta1 (human) with mEosEM fused at the C-terminus.DepositorInsertImportin Beta (KPNB1 Human)
UseTags6xHis and mEos4b (EosEM)ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Pchi-HMG
Plasmid#231540PurposeExpresses the HMG of Pchi Sox with a 6xHis tag for protein purificationDepositorInsertSox HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Salhel-HMG
Plasmid#231539PurposeExpresses the HMG of Salhel Sox-I with a 6xHis tag for protein purificationDepositorInsertSox-I HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Myflu-POU
Plasmid#231542PurposeExpresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purificationDepositorInsertPOU DBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Salhel-POU
Plasmid#231541PurposeExpresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purificationDepositorInsertPOU DBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only