We narrowed to 19,379 results for: INO

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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCH68 (dAsCas12a-3xKRAB piggyBac)

Plasmid

#217334

Purpose

piggyBac expression of dAsCas12a-3xKRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

dAsCas12a

Use

CRISPR

Tags

nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

Expression

Mammalian

Mutation

E993A

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001

Plasmid

#139311

Purpose

Recruitable PI4KA catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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BII-TOP-tdT

Plasmid

#133395

Purpose

Piggybac vector for canonical Wnt signaling reporter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

tdTomato-NLS

Expression

Mammalian

Mutation

N/A

Promoter

SuperTOPFlash

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

cspA

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pClodANL-FGF4

Plasmid

#107874

Purpose

Bacterial expression of FGF4-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF4 (FGF4 Synthetic)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSUB11

Plasmid

#169699

Purpose

Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

Depositor

Nara Figueroa

Article

Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

Insert

3xFLAG tag adjacent to FRT-flanked aph (KanR) gene

Tags

3xFLAG tag

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPSup_35Senh(fwd)_35Spr+synJ

Plasmid

#149422

Purpose

Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Insert

35S core enhancer

Expression

Plant

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PLG-bio-His

Plasmid

#53427

Purpose

Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PLG (PLG Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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Myo1E-pEGFP

Plasmid

#124658

Purpose

Mouse myosin-1e fused with EGFP

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Myo1e (Myo1e Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-C1-GOLGA4-GRIP

Plasmid

#220068

Purpose

overexpreeses the TGN protein GRIP with a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

GOLGA4-GRIP (GOLGA4 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH623

Plasmid

#171633

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-non-targeting
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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