We narrowed to 149,122 results for: ins;

1. pcDNA3.1-RaACR_687-EYFP

   Plasmid

   #111077

   Purpose

   Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RaACR_687
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPI452

   Plasmid

   #113203

   Purpose

   Inducible expression vector (dox-on) N-terminal mScarlet tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum inducible expression vec…

   Tags

   mScarlet

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMBA323

   Plasmid

   #214751

   Purpose

   To create delta(glmS,nadE) working strains and maintaining their viability

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-NBid-PhoCl1-CBid

   Plasmid

   #164050

   Purpose

   Optogenetic manipulation of cell apoptosis using PhoCl1

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NBid-PhoCl1-CBid
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NpT7-5-ERK1

   Plasmid

   #39229

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   ERK1 (MAPK3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

7. MAGE-A3Pro pGL4basic

   Plasmid

   #78138

   Purpose

   luciferase reporter plasmid

   Depositor

   Heidi Schwarzenbach

   Article

   Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

   Insert

   MAGE-A3 (MAGEA3 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   contains -418/+12 of promoter

   Promoter

   MAGE-A3

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAS-hActin-luc2

   Plasmid

   #48081

   Purpose

   Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Luciferase
   

   Expression

   Mammalian

   Mutation

   removed hsp70 promoter, inserted amplified 615 bp…

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pDM1513

   Plasmid

   #108998

   Purpose

   safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Safe haven knock-in plasmid for the act5 locus, n…

   Tags

   GFP

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACS2 Truncated

    Plasmid

    #13748

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 39–682

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-iLID-mCherry-RAB5

    Plasmid

    #174624

    Purpose

    Optogenetic coupling to early endosomes (RAB5) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB5 (RAB5A Human, Synthetic)
    

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB5: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1 PIK3R1-Ni

    Plasmid

    #17744

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1-Ni (PIK3R1 Human)
    

    Expression

    Insect

    Mutation

    Partial cDNA

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDM1515

    Plasmid

    #109000

    Purpose

    safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, c…

    Tags

    GFP

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPI458

    Plasmid

    #113212

    Purpose

    safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, c…

    Tags

    mScarlet

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDM1514

    Plasmid

    #108999

    Purpose

    safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, n…

    Tags

    mCherry

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pr-deGFP-MGapt

    Plasmid

    #67734

    Purpose

    OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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