We narrowed to 13,810 results for: OVA

Showing: 7201 - 7230 of 13810 results

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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE3_IVT

Plasmid

#177015

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE3

Use

CRISPR; Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102G

Plasmid

#112167

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-Merlin-Flag-HA

Plasmid

#27103

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Tags

Flag and HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pHSP70B-CP6-pac

Plasmid

#165877

Purpose

Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pOCC114

Plasmid

#118904

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with TEV protease, two glycines

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mOrange

Plasmid

#134298

Purpose

doxycycline-inducible expression of mouse mOrange in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

mOrange

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.R205A

Plasmid

#112178

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Expression

Mammalian

Mutation

R205A (no GABA binding)

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSC201

Plasmid

#129387

Purpose

A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

Depositor

Silvia Cardona

Article

Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

Insert

oriR6K and oriT

Expression

Bacterial

Mutation

N/A

Promoter

N/A

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBSc polyA + Shal1

Plasmid

#16196

Depositor

Larry Salkoff

Article

Wei et al Science. 1990 May 4. 248(4955):599-603.

Insert

Drosophila melanogaster Shaker cognate l (Shal Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L

Plasmid

#112168

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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mNG-mTq2

Plasmid

#124217

Purpose

Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-mTq2

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPaxillin-TagRFP675-N1

Plasmid

#44276

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

chicken paxillin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Oct. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId2sp

Plasmid

#16044

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id2 (Id2 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 1-195

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pSopB2xHA

Plasmid

#112513

Purpose

PsopB driven sopB-2xHA and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB-2xHA/sigE operon

Tags

2xHA

Expression

Bacterial

Mutation

SopB C-terminal 2xHA

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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RnAT1aR-mCherry

Plasmid

#137782

Purpose

Visualization of At1aR

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat AT1a receptor (Agtr1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBS B2rescue A70D

Plasmid

#78658

Purpose

Generates B2 inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

B2rescue cassette

Expression

Mammalian

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pYIAL31

Plasmid

#60807

Purpose

used to make mutations in yeast POL1

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

Pol1 (POL1 Budding Yeast)

Expression

Yeast

Mutation

N-terminal part only

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-scFv

Plasmid

#78900

Purpose

Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scFV-VP64

Use

CRISPR

Tags

GFP

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

Plasmid

#172224

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
XE152 GFP-XDpr-CS2+

Plasmid

#16770

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

GFP-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pER8 cHA-AIP2

Plasmid

#118687

Purpose

Plant inducible expression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AIP2 (AT5G20910 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

LexA

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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