We narrowed to 4,893 results for: PIR

1. pCMV-dLbCpf1-BE-YEE

   Plasmid

   #154148

   Purpose

   Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YEE (dCas12a-BE-YEE)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

   Promoter

   CMV

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1841

   Plasmid

   #154338

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. KHBD00189

   Plasmid

   #34341

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG2988 (ems Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Str-Endo-KKXX_SBP-EGFP-LIMP2

   Plasmid

   #222323

   Purpose

   Synchronize the trafficking of LIMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL_SBP-mCherry-CCR1

   Plasmid

   #222311

   Purpose

   Synchronize the trafficking of CCR1 from the ER.

   Depositor

   Franck Perez

   Article

   Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

   Insert

   Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-Ii_SBP-mCherry-Golgin84

   Plasmid

   #65304

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SynaptoTAG2

   Plasmid

   #175275

   Purpose

   AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
   

   Use

   AAV

   Tags

   EGFP and tdTomato (P2A cleavage)

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-TagRFP-T_A1aY1

    Plasmid

    #158754

    Purpose

    Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_TagRFP-T_A1aY1
    

    Tags

    6x-Histidine tag and TagRFP-T

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-Ii_SBP-EGFP-Golgin84

    Plasmid

    #65303

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SBP-GFP-Tac-TC

    Plasmid

    #162506

    Purpose

    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP and SBP

    Expression

    Mammalian

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-GABRA1(A322D)-IRES2-EGFP

    Plasmid

    #170821

    Purpose

    human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

    Depositor

    Tingwei Mu

    Article

    Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

    Insert

    Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
    

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    change Alanine 322 to Aspartate

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET32a-hEPR

    Plasmid

    #199233

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Proepiregulin preproprotein (EREG Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mini-Ii-Str_SBP-EGFP-LIMP2

    Plasmid

    #222324

    Purpose

    Synchronize the trafficking of LIMP2 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid

    #117274

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV and IRES

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFR-ET1

    Plasmid

    #44185

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    EGFR-ET1 (EGFR Human)
    

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    I104V and D254N relative to NP_005219.2

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Opto-GPR55

    Plasmid

    #106026

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR55 (GPR55 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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