We narrowed to 15,882 results for: LEA

Showing: 4821 - 4840 of 15882 results

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

No tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shTBK1_3185

Plasmid

#167294

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA TBK1 (TBK1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA Ago2 corrected

Plasmid

#10822

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 2 (AGO2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

3 silent mutations in orf

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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CRY2olig-PR

Plasmid

#218917

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-PR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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CRY2olig-GR

Plasmid

#218919

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-GR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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PS Intein NLS-NES

Plasmid

#242021

Purpose

Blue-light-induced reconstitution of mCherry and its relocalization to the nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein NLS-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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