We narrowed to 43,163 results for: Ina

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pCEP4-FLAG-TAPBPR-TM-VC

Plasmid

#135499

Purpose

Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Tags

Luminal/Extracellular FLAG epitope tag, Signal pe…

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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iMb2-Control-Mosaic (SO273)

Plasmid

#99750

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS-ERK-YFP

Plasmid

#42076

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila ERK ortholog (rl Fly)

Tags

YFP

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
HA-HIF2α P405A/P531A/N847A-pBabe-Puro

Plasmid

#25956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/stSpike-puro

Plasmid

#161794

Purpose

Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 secretable trimeric Spike

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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FGFR1_HUMAN_D0

Plasmid

#79719

Purpose

This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FGFR1 (FGFR1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-TEAD4

Plasmid

#128335

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Insert

TEAD4 (TEAD4 Human)

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP10-14-SEPT7

Plasmid

#180341

Purpose

mammalian expression of human SEPT7 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Flpo Flp-in replacement vector

Plasmid

#61572

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Flpo

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

Plasmid

#135176

Purpose

AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Tetsuo Yamamori

Article

Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

Inserts

hChR2
TRE3G

Use

AAV and Cre/Lox

Tags

EYFP (C terminal on insert)

Expression

Mammalian

Mutation

E123T and T159C

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

Plasmid

#25948

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Tre-Tight ifgMosaic Donor vector (LH121)

Plasmid

#99630

Purpose

To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Mutation

(Please see depositor comments below)

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP10

Plasmid

#180343

Purpose

mammalian expression of human SEPT9_i1 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-R617M,K618M,K632M,K633M

Plasmid

#115778

Purpose

Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

Androgen Receptor (AR Human)

Expression

Mammalian

Mutation

R617M, K618M ,K632M, K633M, insertion of Q81 and …

Promoter

CMV

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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CHK2_HUMAN_D0

Plasmid

#79715

Purpose

This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CHK2 (CHEK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
W118-1_hRSK3(RPS6KA2)_K91A_K444A

Plasmid

#180385

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2_K91A/K444A (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K91A/K444A

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV superYFP-AURKA K162M-mTurq2

Plasmid

#157773

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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