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pscAAV-GFP

Plasmid

#32396

Depositor

John T Gray

Article

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

Plasmid

#133560

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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LV TRE-GFP-TMD-DLL1

Plasmid

#194936

Purpose

LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

eGFP-(PDGFR)TMD-rDLL1 ICD (WT)

Use

Lentiviral

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Article

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1 (1140H)

Plasmid

#174849

Purpose

Expression of DDB1

Depositor

Helen Morrison

Insert

DDB1 (DDB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.5

Plasmid

#111058

Purpose

Expresses dLight1.5 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.5

Tags

Flag tag

Expression

Mammalian

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBUE411-B

Plasmid

#198234

Purpose

pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Article

Chen et al bioRxiv 2022.09.02.506370

Insert

pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

PLTP

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Centrin2 (Nigg UK185)

Plasmid

#41147

Depositor

Erich Nigg

Article

Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

Insert

Centrin-2 (CETN2 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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Anti-GST [N100/13R ]

Plasmid

#188214

Purpose

Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX 4T-1 GST-Ago2 S387A

Plasmid

#60656

Purpose

Expresses GST-Ago2 S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

GST

Expression

Bacterial

Mutation

S387A

Promoter

tac

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DAAO

Plasmid

#60344

Purpose

Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

Depositor

Vsevolod Belousov

Article

Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

Insert

DAAO

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTRIP-UbC-Blast-2A-STING-mNeonGreen

Plasmid

#227185

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

Depositor

Nir Hacohen

Article

Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

UbC

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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3xnls-mNeonGreen

Plasmid

#98875

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde and AAV1

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158741

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/MI/45/15 H1N1

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/SCAF4-FLAG

Plasmid

#122469

Purpose

Dox-inducible FLAG (C-terminal tag) SCAF4 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 (SCAF4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF1, delta ZF2

Plasmid

#84027

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-58; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF2

Plasmid

#84026

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 35-58; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta ZF1

Plasmid

#84025

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-34; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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SPACE (pRZ1813)

Plasmid

#140242

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE

Expression

Mammalian

Mutation

V82G in TadA*, D10A in Cas9, R187W in pmCDA1

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHM053Env

Plasmid

#139019

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein (env HIV-1)

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ delta GLEBS

Plasmid

#84028

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 357−373; Q394R

Promoter

UCOE / SFFV

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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