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Plasmid#54120PurposeLocalization: Membrane Adaptor, Excitation: 487, Emission: 509DepositorInsertHomer1 (Homer1 Rat)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
PRS_8-jGCaMP7b
Plasmid#192594PurposeAAV-vector for fluorescent calcium measurements from noradrenergic neuronsDepositorInsertjGCaMP7b
UseAAVTagsExpressionMammalianMutationPromoterPRSX8Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-N-HA-Pcdh15_mini_V4
Plasmid#199185PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.DepositorInsertHA-mmPcdh15_mini_V4-CD2
UseAAVTagsHuman influenza hemagglutinin (HA)ExpressionMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1456
Plasmid#143811PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTFEC (TFEC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.0
Plasmid#102548Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG-ABEmax
Plasmid#124163PurposeA-to-G base editorDepositorInsertTadA-TadA(evo)-Cas9-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-eGFP-Z1
Plasmid#135049PurposeEnhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla
Plasmid#60510PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)
Plasmid#101178PurposeHuman expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/Q695A/H698APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-emiRFP670-P2A-GFP
Plasmid#197160PurposeExpresses the protein of emiRFP670-P2A-GFP in mammalian cellsDepositorInsertemiRFP670-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Beta-Catenin-20
Plasmid#55001PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorInsertBeta-Catenin (Ctnnb1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
UbcH5c WT pET28a
Plasmid#12643DepositorInsertUbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-IRES-Puro
Plasmid#58970Purposenoninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorTypeEmpty backboneUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS1-hChR2-tBFP
Plasmid#178706PurposeAAV vector for transgene expression of hChR2-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)DepositorInserthChR2-tBFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVP073
Plasmid#222076PurposeeforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP074
Plasmid#222077PurposeeforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP075
Plasmid#222078PurposeAeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSlick-Neo-RECK
Plasmid#121986PurposeTetracycline-inducible expression of untagged full-length RECKDepositorInsertRECK (RECK Human)
UseLentiviralTagsExpressionMutationPromoterTight TRE promoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-UBC-DSRED-LUC-EF1 Hygro
Plasmid#129437Purposeexpress dsred-luc chimeraDepositorInsertdsred-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
cyto-Ruby3-iATPSnFR1.0
Plasmid#102551Purposeexpresses cytoplasmic ATP sensor in mammalian cells with red reference proteinDepositorInsertRuby3-iATPSnFR1.0
UseTagsRuby3ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-mGold
Plasmid#157996PurposeExpression of mGold in mammalian cellsDepositorInsertmGold
UseTagsExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterActin promoter with CMV enhancerAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
mIL12-N1
Plasmid#123139PurposeMammalian expression of murine cytokine IL-12 under CMV promoterDepositorInsertMurine IL12 tandem p40 p35
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTI825
Plasmid#216633PurposeCal-ID (V5) for AAV productionDepositorInsertCal-ID
UseAAVTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only