We narrowed to 16,478 results for: por

1. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

   Plasmid

   #192452

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   3x Nuclear localization signal (NLS) and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBeast-pYjjZ-RBS- sfGFP

   Plasmid

   #190054

   Purpose

   GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPgiCas13b-gRNA-1

   Plasmid

   #184834

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPgiCas13b-gRNA-NT

   Plasmid

   #184835

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNHT7::UMSBP::sNluc2_ORF1

   Plasmid

   #186765

   Purpose

   An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNHT7::ENO::sNluc2

   Plasmid

   #186770

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNHT7::RPL15::sNluc0

   Plasmid

   #186771

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNHT7::RPL15::hNluc

   Plasmid

   #186772

   Purpose

   An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNHT7::YB1::hNluc

   Plasmid

   #186774

   Purpose

   An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAPDH::sNluc1

    Plasmid

    #187221

    Purpose

    A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Overexpression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNHT7::YB1::sNluc1

    Plasmid

    #186754

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNHT7::ENO::sNluc1

    Plasmid

    #186755

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNHT7::RPL10::sNluc1

    Plasmid

    #186756

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNHT7::RPL15::sNluc1

    Plasmid

    #186757

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNHT7::YB1::sNluc0

    Plasmid

    #186758

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNHT7::YB1::sNluc2

    Plasmid

    #186759

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNHT7::YB1::sNluc3

    Plasmid

    #186760

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNHT7::YB1::sNluc4

    Plasmid

    #186761

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNHT7::YB1::sNluc5

    Plasmid

    #186762

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNHT7::RPL15::sNluc2_STOP

    Plasmid

    #186767

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Premature stop codon at residue 16

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNHT7::RPL15::sGluc

    Plasmid

    #186769

    Purpose

    An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET24a-LaM4-2xTS

    Plasmid

    #162779

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFBP-2_6m.mutant

    Plasmid

    #162842

    Purpose

    Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6m_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-Hygro-EF1A-KR-Linker-eGFP

    Plasmid

    #176066

    Purpose

    EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    KR
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiCRISPRv1_COX4

    Plasmid

    #177983

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting COX4

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting COX4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

    Plasmid

    #154183

    Purpose

    expresses squirrel monkey CHMP3(155) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3(155)
    

    Use

    Tags

    FLAG, Strep-Tag II, and preScission site

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 155

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWZL N-Myc-hPOT1_K90E-IRES_hygro

    Plasmid

    #166407

    Purpose

    Express hPOT1 K90E in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K90E

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MPC027: pAAV-CAG-FLEX-sNovArch-PP-Citrine_KV2.1TS-ER2 (soma targeting)

    Plasmid

    #153196

    Purpose

    encodes pAAV soma targeted NovArch

    Depositor

    Adam Cohen

    Article

    Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

    Insert

    sNovArch
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge