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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g2 LentiCRISPRv2-mCherry

Plasmid

#218663

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g1 LentiCRISPRv2-mCherry

Plasmid

#218662

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB10769

Plasmid

#212694

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Expression

Yeast

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12719

Plasmid

#212702

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB11787

Plasmid

#212695

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN]

Plasmid

#196841

Purpose

Express NES-PUFc-FseI[ADAR2-DD-splitN]

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

NES-PUFc-FseI[ADAR2-DD-splitN]

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pzk491-pmax-NES-PUFc-FseI[ADAR2-DD-splitC]

Plasmid

#196842

Purpose

Express NES-PUFc-FseI[ADAR2-DD-splitC]

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

NES-PUFc-FseI[ADAR2-DD-splitC]

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA)

Plasmid

#196827

Purpose

Express dpdpCas13b with AAAA mutation

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Tags

3XFLAG

Expression

Mammalian

Mutation

K367A, D369A and K370A

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB6637

Plasmid

#212696

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8860

Plasmid

#212697

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8856

Plasmid

#212701

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8866

Plasmid

#212703

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB6630

Plasmid

#212700

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB6638

Plasmid

#212698

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAC13

Plasmid

#197407

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pVS1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pVS1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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