We narrowed to 5,415 results for: PEP;

1. Galectin3-CD4d3+4-bio

   Plasmid

   #73128

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

   Plasmid

   #184335

   Purpose

   Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha-GPI (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. psicheck-2-LPPRC-mod

   Plasmid

   #48168

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   A/T mutation in the ATG of the uORF

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-CNTF

   Plasmid

   #195338

   Purpose

   Expression of CNTF in mammalian cells

   Depositor

   Kevin Park

   Article

   Yungher et al Gene Ther. 2015 Oct;22(10):811-21. doi:

   Insert

   Ciliary neurotrophic factor (CNTF Human)
   

   Use

   AAV

   Tags

   NGF signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   UbC promoter with beta-globin intron

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS-foxc1a-2a-nlstdTomato

   Plasmid

   #132970

   Purpose

   full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   tdTomato
   foxc1a (foxc1a Zebrafish)
   

   Use

   Tags

   NLS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RH2b-bio

   Plasmid

   #47778

   Purpose

   Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH2b (partial)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

   Plasmid

   #194459

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pf92-bio

   Plasmid

   #47728

   Purpose

   Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised Pf92
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKTop::tse5-CT (1169-1281)

    Plasmid

    #192955

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1281)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1281)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

    Plasmid

    #166018

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO/SH/GW-Mst2

    Plasmid

    #84300

    Purpose

    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

    Depositor

    Jonathan Chernoff

    Article

    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    HA, SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Cntn6-AP-His

    Plasmid

    #71944

    Purpose

    Expresses the extracellular region of the Contactin 6 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn6 (Cntn6 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. psicheck-2-LPPRC

    Plasmid

    #48167

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Promoter

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid

    #112712

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    EGFP
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), biotin…

    Expression

    Mammalian

    Mutation

    Nucleotide sequence optimized for synthetic gene …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNUT N6His Y95F/Y188F hTFNG

    Plasmid

    #69601

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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