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We narrowed to 10,761 results for: T7

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Showing: 9801 - 9840 of 10761 results

  1. pTYB-pLAC-L108S/L109S/F112S

    Plasmid
    #48730
    Purpose
    Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine
    Depositor
    Gordon Laurie
    Article
    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6
    Insert
    Lacritin (LACRT Human)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    Leucine 108 and 109 to Serine; phenylalanine 112 …
    Promoter
    T7
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pTYB1-pLAC-N71

    Plasmid
    #48720
    Purpose
    Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus
    Depositor
    Gordon Laurie
    Article
    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6
    Insert
    Lacritin (LACRT Human)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    Deleted first 71 amino acids of mature lacritin w…
    Promoter
    T7
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pJK048

    Plasmid
    #75230
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    75230-plasmid-map-sequence-id-142240

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  15. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

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  19. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  22. CCR7-DuET

    Plasmid
    #213203
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR7 (CCR7 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213203-plasmid-map-sequence-id-430766

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  27. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  37. PKA-N-HiBiT

    Plasmid
    #211377
    Purpose
    Bacterially expressed HiBiT-PKA for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    211377-plasmid-map-sequence-id-417163

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Showing: 9801 - 9840 of 10761 results