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We narrowed to 21,452 results for: SPR

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Showing: 9841 - 9880 of 21452 results

  8. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  20. PB-TAC-OK+9MS-Cas9

    Plasmid
    #120354
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120354-plasmid-map-sequence-id-246101

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  21. TR- ATG-HP-GFP

    Plasmid
    #80592
    Purpose
    Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    GFP
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Inserted 30 nt hairpin which is targeted by Csy4 …
    Promoter
    CBA
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    80592-plasmid-map-sequence-id-151877

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  24. pDIRECT_21D

    Plasmid
    #91131
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91131-plasmid-map-sequence-id-182255

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  29. ICBE

    Plasmid
    #247331
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247331-plasmid-map-sequence-id-496500

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  30. RUBY-ABE#2

    Plasmid
    #232176
    Purpose
    T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.
    Depositor
    Yiping Qi
    Article
    Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265
    Insert
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
    Use
    CRISPR
    Tags
    3xflag and NLS
    Expression
    Plant
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    232176-plasmid-map-sequence-id-459941

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  31. pDM052

    Plasmid
    #216814
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216814-plasmid-map-sequence-id-429539

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  32. hfDECOR-F148A

    Plasmid
    #219546
    Purpose
    Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-F148A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed TadA8e Phenylalanine 148 to Alanine
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219546-plasmid-map-sequence-id-443106

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  33. hfDECOR-V106G

    Plasmid
    #219547
    Purpose
    Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Insert
    hfDECOR-V106G
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed TadA8e Valine 106 to Glycine
    Promoter
    CMV
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219547-plasmid-map-sequence-id-443107

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  35. pDonor-LoxP

    Plasmid
    #219436
    Purpose
    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens
    Depositor
    Sheng Yang
    Article
    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-
    Insert
    two transposon ends, LoxP-terminator-LoxP gene cassette
    Expression
    Bacterial
    Available Since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219436-plasmid-map-sequence-id-435435

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Showing: 9841 - 9880 of 21452 results