We narrowed to 17,204 results for: puro

1. pMXs-IP-EGFP-ULK1

   Plasmid

   #38193

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMN-009

   Plasmid

   #195413

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAC026

   Plasmid

   #227598

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SETMAR-Cas9

   Plasmid

   #186701

   Purpose

   Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETMAR-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-c-Myc-IP

   Plasmid

   #15921

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB_TRE_sg30-PDGFR_EF1a_mCherry

   Plasmid

   #186309

   Purpose

   Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg30-PDGFR
   mCherry
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIP-caTfeb

   Plasmid

   #79013

   Purpose

   For mammalian expression of a shortened, constitutively active version of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (TFEB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Removed first ~400 bp from 5' end (N-terminu…

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pMRX-IP-3xFLAG-NCOA4 (dIDR2)

   Plasmid

   #192811

   Purpose

   Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of the IDR2

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AsCas12a

    Plasmid

    #188492

    Purpose

    Expresses FLAG tagged AsCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMRX-IP-3xFLAG-NCOA4 (dN)

    Plasmid

    #192808

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMRX-IP-3xFLAG-NCOA4 (dM)

    Plasmid

    #192810

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the Middle domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRX-IP-3xFLAG-NCOA4 (dIDR1)

    Plasmid

    #192809

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the IDR1

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IP-3xFLAG-NCOA4 (dC)

    Plasmid

    #192812

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IP-3xFLAG-NCOA4 (I56E)

    Plasmid

    #192813

    Purpose

    Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    I56E mutation in the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRX-IP-3xFLAG-NCOA4 (L63R)

    Plasmid

    #192814

    Purpose

    Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L63R mutation in the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2-scr

    Plasmid

    #169795

    Purpose

    Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Scrambled guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIQmate

    Plasmid

    #216240

    Purpose

    All-in One IQ-Cumate transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pWXLd-RIEP-CeNLuc

    Plasmid

    #135934

    Purpose

    Expresses the CeNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

    Plasmid

    #159455

    Purpose

    Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p8092 LentiCRISPR v2 sgNT-1

    Plasmid

    #163315

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgNT-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1P shACO1

    Plasmid

    #169884

    Purpose

    Suppress ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against ACO1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SP-GFP-CD274-UTR

    Plasmid

    #121142

    Purpose

    Mammalian expression of GFP tagged CD274 with its own 3'UTR

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    CD274 with its own 3'UTR (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Flag pLJM1 Rap2A

    Plasmid

    #19311

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RAP2A, member of RAS oncogene family (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLJM1 RagA

    Plasmid

    #73031

    Purpose

    Expresses human RagA from a CMV promoter for stable expression in mammalian cells

    Depositor

    David Sabatini

    Article

    Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

    Insert

    rragA (RRAGA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFLAG/HA-DGCR8

    Plasmid

    #10921

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-EF1a-hMLH1dn-eGFP

    Plasmid

    #191104

    Purpose

    Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PB-TO-hNGN2-BSD-mApple

    Plasmid

    #182308

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    hNGN2 (NEUROG2 Human)
    bsd-T2A-mycNLS-mApple
    

    Use

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMRXIP-GFP-YKT6

    Plasmid

    #116946

    Purpose

    Expressing GFP-YKT6 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    Synaptobrevin homolog YKT6 (YKT6 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Fuw CBX8

    Plasmid

    #121111

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pD2529-CAG-HA-mouse LRRC33

    Plasmid

    #180259

    Purpose

    Mouse full length LRRC33 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    LRRC33 (Nrros Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiCRISPRv2-ACTB-C1

    Plasmid

    #169796

    Purpose

    Expresses Cas9 and guide RNA for ACTB gene in human

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLKO human ULK1 shRNA 8

    Plasmid

    #27633

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    human ULK1 shRNA 8
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDA007

    Plasmid

    #131380

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Use

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mNeonGreen-Smad3

    Plasmid

    #172098

    Purpose

    Expresses fluorescently tagged human Smad3 in mammalian cells.

    Depositor

    Lea Goentoro

    Article

    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Smad 3 (Smad3 Mouse)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    C49S mutation in mCerulean

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge