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pGWB527

Plasmid

#74869

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLTR PKC delta

Plasmid

#8419

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
CLM RP HTR

Plasmid

#24430

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGWB525

Plasmid

#74867

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-Dusp6DM_S159/197A

Plasmid

#67684

Purpose

3' entry clone with activated Dusp6 (S159/167A)

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Mutation

S159A, S197A

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB429

Plasmid

#74823

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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R10-3 /pQE-30

Plasmid

#79859

Purpose

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

Depositor

Konstantin Lukyanov

Article

Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

Insert

R10-3

Tags

HisTag

Expression

Bacterial

Mutation

Aequorea victoria green fluorescent protein (avGF…

Promoter

T5 promoter / lac operator

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLTR PKC alpha

Plasmid

#8416

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-PTEN-H123Y

Plasmid

#28299

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant His123Tyr

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pMTH PKC beta II

Plasmid

#8410

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
vsv-CenpB 1-166-Sgo1-GFP

Plasmid

#108495

Purpose

expression of vsv-CenpB-1-166-Sgo1-EGFP

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Desmin/pAS2-1

Plasmid

#97407

Purpose

Desmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

Desmin (DES Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGST-Fah_W

Plasmid

#163089

Purpose

Expressing mouse wildtype FAH in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB526

Plasmid

#74868

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGL1

Plasmid

#48741

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to -141 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to -141)

Use

Luciferase

Mutation

truncated promoter region (see pGL6)

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTre2tet)::Fbxl21

Plasmid

#51890

Purpose

expression of Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV tet

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
B. fragilis mRNA toehold switch sensor

Plasmid

#110706

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. fragilis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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