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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,332 results

Showing: 10001 - 10040 of 159332 results

  3. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  7. pCALNL-DsRed

    Plasmid
    #13769
    Purpose
    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    DsRed2
    Use
    Cre/Lox; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13769-plasmid-map-sequence-id-7758

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  10. mPA-GFP-CD81-10

    Plasmid
    #57124
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    CD81 (CD81 Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  12. pHB-4

    Plasmid
    #140957
    Purpose
    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    140957-plasmid-map-sequence-id-334294

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  17. Mito-SNAP

    Plasmid
    #206480
    Purpose
    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression
    Depositor
    Erika Holzbaur
    Article
    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7
    Insert
    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    Use
    Tags
    SNAPf
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    206480-plasmid-map-sequence-id-435222

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  21. PS-CFP2-Tubulin-6

    Plasmid
    #57251
    Purpose
    Localization: Microtubules, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    NM_006082.2 (TUBA1B Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. TFORF0702

    Plasmid
    #144361
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR2 (NCOR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144361-plasmid-map-sequence-id-353414

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  33. tdTomato-C1

    Plasmid
    #54653
    Purpose
    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54653-plasmid-map-sequence-id-450928

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  36. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170442-plasmid-map-sequence-id-337888

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  40. HSPA9A

    Plasmid
    #42469
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA9A (HSPA9 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10001 - 10040 of 159332 results