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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpG-P2A-EGFP (RTW4177)

Plasmid

#139988

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pX458M-53BP1-DN1S

Plasmid

#131045

Purpose

Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

Depositor

Punam Malik

Article

Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

Insert

SpCas9-53BP1-DN1S (TP53BP1 Human)

Use

CRISPR

Tags

GFP and SpCas9

Expression

Mammalian

Mutation

Only expressing residues 1231-1644 of complete 53…

Promoter

CBH

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV/L7-6-GFP-WPRE

Plasmid

#126462

Purpose

An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

Depositor

Hirokazu Hirai

Article

Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

Insert

L7-6, Purkinje cell specific promoter

Use

AAV

Expression

Mammalian

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

Plasmid

#118154

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

EF1a core and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-sadCas9-VP64

Plasmid

#115790

Purpose

An AAV vector expressing S. aureus dCas9 fused to VP64.

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

Sa-dCas9

Use

AAV

Tags

NLS and NLS-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Mutation

D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (mCherry)

Plasmid

#102945

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Chicken, Synthetic)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
pXPR_047

Plasmid

#107145

Purpose

measures Cas9 activity

Depositor

David Root

Insert

GFP + sgRNA for GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Expression

Mammalian

Promoter

mDLX5/6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP

Plasmid

#75452

Purpose

Plasmid for constitutive expression of gfp

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Article

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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