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pGEX-4T-1_RAF1 (51-131)

Plasmid

#119218

Purpose

purification of GST-RAF1 RBD from E. coli

Depositor

Helen Morrison

Article

Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

Insert

RAF1 aa 51-131 (RAF1 Human)

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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mScarlet-PPM1H

Plasmid

#233585

Purpose

Expresses PPM1H in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

PPM1H (PPM1H Human)

Tags

mScarlet

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-KATNA1

Plasmid

#180650

Purpose

Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GGACAGCACUCCCUUGAAA

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

Plasmid

#209194

Purpose

Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

Depositor

Larry Zweifel

Article

Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

Insert

Kcnq3 (KCNQ3 Human)

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

R230 to C230

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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mRFP-PA-GFP-RhoAQ63L

Plasmid

#187282

Purpose

Express pmRFP-PAGFP-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

RhoA (RHOA Human)

Tags

mRFP-PAGFP

Expression

Mammalian

Mutation

RhoA Q63L

Promoter

CMV, T7

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT_KCNQ3

Plasmid

#173163

Purpose

To generate Flp-In CHO-KCNQ3 stable cells

Depositor

Al George

Article

Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti.CMV/TO_PRKAA2

Plasmid

#74447

Purpose

Lentiviral expression vector encoding untagged WT human AMPK alpha 2

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

AMPK alpha 2 (PRKAA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
plenti-UBC- IRF4-3xHA-pGK-PUR

Plasmid

#107386

Purpose

Lentiviral expression of IRF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPR

Plasmid

#38067

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)

Tags

FLAG, HA, and His

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

Plasmid

#166794

Purpose

Mammalian cell expression vector for Flag-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1

Plasmid

#60540

Purpose

Donor vector for human MLK1

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-TetR

Plasmid

#103798

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pN1-RnKIF5C(1-560)-Halo-Flag

Plasmid

#188071

Purpose

Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

Depositor

Kristen Verhey

Article

Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

Insert

rat KIF5C (Kif5c Rat)

Tags

Flag and Halo

Expression

Mammalian

Mutation

aa1-560

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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CIB-VP64 (B1016)

Plasmid

#92037

Purpose

Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1-VP64 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 with VP64 activation domain.…

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 EDD

Plasmid

#37190

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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