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We narrowed to 18,247 results for: multi

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Showing: 5101 - 5120 of 18247 results

  3. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Publication
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  7. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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  10. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Publication
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  13. p11d-tRPA-32Ala9

    Plasmid
    #102616
    Purpose
    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).
    Depositor
    Marc Wold
    Inserts
    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    Expression
    Bacterial
    Mutation
    with an alanine substituted phosphorylation domain
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102616-plasmid-map-sequence-id-197482

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  16. pKD233.7-3

    Plasmid
    #90955
    Purpose
    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Publication
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Expression
    Bacterial
    Promoter
    Bba_J23114, PltetO-1, and PttrB185-269
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90955-plasmid-map-sequence-id-178326

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  18. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68417-plasmid-map-sequence-id-120195

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Showing: 5101 - 5120 of 18247 results