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We narrowed to 21,452 results for: SPR

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Showing: 10281 - 10320 of 21452 results

  5. pCAG-B2UP49_GFP

    Plasmid
    #103071
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103071-plasmid-map-sequence-id-201896

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  7. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

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  8. AAEL007584-Cas9

    Plasmid
    #100706
    Purpose
    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL007584 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100706-plasmid-map-sequence-id-193543

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  12. pSA091 PSMD14_IVS2-Cas9-BFP

    Plasmid
    #249141
    Purpose
    Overexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PSMD14_IVS2-Cas9-TagBFP (PSMD14 Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    PSMD14 IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249141-plasmid-map-sequence-id-496524

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  13. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

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  17. pMMACE DEST CMV H2B iRFP

    Plasmid
    #206253
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B iRFP713
    Use
    Recombinant baculovirus production, multimate/gat…
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206253-plasmid-map-sequence-id-406403

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  18. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206259-plasmid-map-sequence-id-406393

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  20. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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  22. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  23. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  25. LentiV_Neo_SIK3_CR_T142Q

    Plasmid
    #108109
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108109-plasmid-map-sequence-id-207842

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  35. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

    Plasmid
    #228726
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D56R
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228726-plasmid-map-sequence-id-454011

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  36. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

    Plasmid
    #228725
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228725-plasmid-map-sequence-id-454009

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  37. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

    Plasmid
    #228730
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D56R
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228730-plasmid-map-sequence-id-454000

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  40. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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Showing: 10281 - 10320 of 21452 results