We narrowed to 22,195 results for: his

1. pET14b-PITPalpha K61A

   Plasmid

   #58258

   Purpose

   bacterial expression of human PITP alpha K61A

   Depositor

   Shamshad Cockcroft

   Article

   Tilley et al Structure. 2004 Feb;12(2):317-26.

   Insert

   PITP alpha (PITPNA Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K61A (abolishes PI binding)

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET15b/GgVcl 1-851 A50I mutation

   Plasmid

   #46174

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

   Insert

   Vcl 1-851 A50I (VCL Chicken)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-851 A50I mutation (talin binding domain mut…

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQTEV-PTK9L

   Plasmid

   #31587

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   PTK9L protein tyrosine kinase 9-like (A6-related protein) (TWF2 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-MRPL15

   Plasmid

   #31345

   Depositor

   Linda Spremulli

   Article

   Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

   Insert

   mitochondrial ribosomal protein L15 mature form (MRPL15 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Has the amino acids in the predicted mature form …

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

5. sankE3_5

   Plasmid

   #24893

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   sank E3_5
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET-sCS4T+N

   Plasmid

   #28255

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   sCS4T+N
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   single chain CSE4-SCM3-H4 with Thrombin site, SCM…

   Promoter

   Available Since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

7. PrbC5

   Plasmid

   #24787

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   Prb
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

   Promoter

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pEBG-hBEN-deltaNLS3

   Plasmid

   #22161

   Depositor

   Ananda Roy

   Article

   Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

   Insert

   hBEN (GTF2IRD1 Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 883-889

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pEBG-hBEN-deltaSS

   Plasmid

   #22162

   Depositor

   Ananda Roy

   Article

   Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

   Insert

   hBEN (GTF2IRD1 Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 897-908

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pEBG-TFII-I L30&37P

    Plasmid

    #22166

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 30 to Proline and changed leucine…

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pEBG-hBEN L38&45P

    Plasmid

    #22182

    Depositor

    Ananda Roy

    Article

    Tantin et al J Biol Chem. 2004 Feb 13. 279(7):5460-9.

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 38 to Proline and changed Leucine…

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pRmHa3-dKDM4A-H195A-HA2FL2

    Plasmid

    #20235

    Depositor

    Jerry Workman

    Article

    Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

    Insert

    dKDM4A (Kdm4A Fly)
    

    Use

    Tags

    FLAG2 and HA2

    Expression

    Insect

    Mutation

    changed His 195 to Ala

    Promoter

    Available Since

    March 19, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pET19b NFRKB 1-101

    Plasmid

    #19787

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    only contains amino acids 1-101

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hsDCX-pHAT

    Plasmid

    #83928

    Purpose

    hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Gary Brouhard

    Article

    Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

    Insert

    human Doublecortin isoform b (DCX Human)
    

    Use

    Tags

    6-His tag and Strep-TagII

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Use

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5/Lac

    Available Since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Use

    Tags

    6xR and His

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac and PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pECIA2

    Plasmid

    #47032

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Fc(human IgG1), HRV 3C Protease Cleavage site, He…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-puro-ARID1A

    Plasmid

    #39478

    Purpose

    Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQTEV-UFD1L

    Plasmid

    #31560

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ubiquitin fusion degradation 1-like (UFD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FL-fibulin-3-V5-lentiviral-GFP

    Plasmid

    #29703

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

29. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

30. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Use

    Tags

    Hexa his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HMBP-3C-STAR.66-285

    Plasmid

    #100094

    Purpose

    expresses MBP-tagged STARD1 protein domain (residues 66-285)

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

    Insert

    STAR protein (STAR Human)
    

    Use

    Tags

    His-tagged MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-synapsin-HaloCaMP1b-EGFP

    Plasmid

    #138328

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1b
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. B23(9-122)

    Plasmid

    #23142

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

    Insert

    B23 core (NPM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-8

    Promoter

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

34. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Cas12aUltra_5XNLS

    Plasmid

    #218775

    Purpose

    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

    Depositor

    Ross Wilson

    Article

    Sahu et al bioRxiv 2024.07.14.603391

    Insert

    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    

    Use

    CRISPR

    Tags

    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

    Expression

    Bacterial

    Mutation

    Nucleotides mutations were made to encode for arg…

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Promoter

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Maria Alvarado-Kristensson, Ana Carrera

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge