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pCAG/GFP

Plasmid

#139980

Purpose

expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Green fluorescent protein

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pmRNA-iSyn-EGFP

Plasmid

#225907

Purpose

In vitro transcription of cap-independently (synthetic IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

PABP motif
HRV-B3-derived synthetic IRES
EGFP
HBA1 UTR

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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JME4390

Plasmid

#129656

Purpose

LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV::hTfR-GFP

Plasmid

#199714

Purpose

Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

hTfR-GFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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WT WWP1

Plasmid

#200085

Purpose

WWP1 in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

WT WWP1 (WWP1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-KIRIN1

Plasmid

#107414

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186A-T2A-BFP

Plasmid

#194932

Purpose

LV construct encoding Y186A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y192A-T2A-BFP

Plasmid

#194933

Purpose

LV construct encoding Y192A mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAMPK alpha2 K45R

Plasmid

#15992

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Mutation

K45R

Promoter

CMV

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-KIRIN1-GR

Plasmid

#107418

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIBY55

Plasmid

#213714

Purpose

Express the affinity sorting tag GST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIDAF

Plasmid

#213713

Purpose

Express the affinity sorting tag GST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET15bHis6Tnp

Plasmid

#79807

Purpose

Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

Depositor

Keji Zhao

Article

Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

Insert

His6Tnp

Tags

His

Expression

Bacterial

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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