We narrowed to 10,847 results for: ENA

1. pHD-SPARC2-S-LexA::p65

   Plasmid

   #133564

   Purpose

   Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

   Plasmid

   #74309

   Purpose

   Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TMEM16F-I521K-peGFP-N1

   Plasmid

   #228588

   Purpose

   Expresses TMEM16F-I521K-peGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   I521K plus a 3 amino acid N-terminal truncation (…

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-Flag-hKlf4

   Plasmid

   #20074

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hKlf4 (KLF4 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

   Plasmid

   #129668

   Purpose

   A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

   Plasmid

   #196678

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIαmut-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-IKZF1-V2-V5

   Plasmid

   #107390

   Purpose

   Mammalian expression of IKZF1-V2-V5

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1-V2 (IKZF1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

   Plasmid

   #127356

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV[Exp]-Neo-EF1A>Tet3G

   Plasmid

   #184379

   Purpose

   The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Tet3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EFS-SpdCas9

    Plasmid

    #210703

    Purpose

    This plasmid express EFS promoter driven human codon optimized SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a core promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Use

    Tags

    Lck and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHD-SPARC2-D-LexA::p65

    Plasmid

    #133562

    Purpose

    Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECE M2-SH3PXD2A WT

    Plasmid

    #69813

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LSB-hsa-miR-218-5p

    Plasmid

    #103357

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-218-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TMEM16F-I611K-peGFP-N1

    Plasmid

    #228581

    Purpose

    Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I611K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YCplac33-NAT_MCART1

    Plasmid

    #140591

    Purpose

    Express MCART1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-3xFLAG-NLS-TPP1

    Plasmid

    #53585

    Purpose

    Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Silent mutation to eliminate PacI site

    Promoter

    CMV

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only