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Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDS282

Plasmid

#34983

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pRiboS EcoHind

Plasmid

#36254

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB8740

Plasmid

#126906

Purpose

Template for PCR amplification of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8741

Plasmid

#126907

Purpose

Template for PCR amplification of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046179

Plasmid

#149059

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CUP Pan1 YFP p316

Plasmid

#15592

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

PAN1 (PAN1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGC146

Plasmid

#19613

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Expression

Worm

Promoter

hsp-16.2

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRSETA-betaFlincG

Plasmid

#19736

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

beta-FlincG

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1673

Plasmid

#112047

Purpose

CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SCT1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-eIF4E

Plasmid

#37228

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pET-TBZ

Plasmid

#28239

Depositor

Yawen Bai

Article

Zhou et al Nat Struct Mol Biol. 2008 Aug . 15(8):86

Insert

single chain Truncated H2B-H2A.Z

Expression

Bacterial

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pRS316-GFP-COF1

Plasmid

#31843

Depositor

David Drubin

Article

Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

Insert

Cofilin (COF1 Budding Yeast)

Tags

GFP (S65T)

Expression

Yeast

Promoter

T7

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCUP1-NMGR

Plasmid

#1211

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)

Tags

GR

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pSB305-K3L-vac-HA

Plasmid

#20054

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Tags

HA

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pRET.IL.IRES-EGFP (No HSV-TK)

Plasmid

#1836

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57 iLOV ANAB

Plasmid

#26770

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Cloning vector

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only