Addgene

We narrowed to 4,271 results for: TIL

Showing: 1251 - 1300 of 4271 results

Results per page:

Sort results by:

pENTR/pSM2(CMV) (w57-1)

Plasmid

#17389

Purpose

Gateway entry vector for miRNA expression under CMV promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnfΔpvc15_pvc12-HiBiT

Plasmid

#198309

Purpose

Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15_pvc12-HiBiT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1

Plasmid

#210552

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT1::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2

Plasmid

#210553

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT2::mCherrySSM

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB-SpCas9

Plasmid

#210533

Purpose

Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

SpCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMulti-sgRNA-LacZ-DsRed

Plasmid

#99914

Purpose

Receptor plasmid for the assembly of multiple sgRNAs with DsRed coexpression

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-Syt::7xHalo7

Plasmid

#87650

Purpose

For creating drosophila transgenics expressing UAS-Syt::7xHalo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::7xHalo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L1-PGK-TKBsd-L2

Plasmid

#141009

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKBsd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCKan-INSyibp-Nef

Plasmid

#134593

Purpose

Low Phosphate inducible Nef

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

HIV Nef protein

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Use

Tags

Expression

Mutation

Promoter

hGFAP

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GALT-APEX2

Plasmid

#215554

Purpose

Stable, inducible expression in Golgi

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

APEX2-NES-GalT-EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOCC82

Plasmid

#118862

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-Ad5DL491-492

Plasmid

#198290

Purpose

Produces PVCs retargeted with non-binding mutant of Ad5 knob

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5DL491-492

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXP CMV/TO LUC (w432-1)

Plasmid

#17476

Purpose

Retroviral Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TC512

Plasmid

#122615

Purpose

Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

Zip(-)::QFAD::NLS::SL2::mCherry

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE001

Plasmid

#48839

Purpose

Provides the rbcS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

rbcS terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO-hCALML3

Plasmid

#47601

Depositor

Emanuel Strehler

Article

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

CALM3 (CALML3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

tet-inducible CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS

Plasmid

#90996

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV-SS-antiSunTag-TM

Plasmid

#198306

Purpose

Displays anti-SunTag scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiSunTag-TM

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pF hPGK mc2 IRES hygro

Plasmid

#206238

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS014

Plasmid

#65467

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

Ntag-beta galactosidase

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

pl-TetO

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
TwinStrep-SUMO-Pdp1

Plasmid

#198320

Purpose

For affinity purification of Pdp1 payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pdp1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-V5-2-Ubc9 (w345-2)

Plasmid

#17407

Purpose

Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

Ubc9 and V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSXND1-hPPT1

Plasmid

#85817

Purpose

Expression of hPPT in mammalian cells

Depositor

Sandra Hofmann

Article

Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

Insert

human PPT1 (PPT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only
pTK105

Plasmid

#59562

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn EYFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pf CAG mc2 IRES blast

Plasmid

#206235

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Article

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIN X2/pSM2(CMV/TO) empty (w334-1)

Plasmid

#17488

Purpose

Retroviral Empty control vector, miRNA (CMV/TO), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pf CAG mc2 IRES puro

Plasmid

#206233

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQF-mNGsacB

Plasmid

#203673

Purpose

pQF with mNeonGreen and sacB under control of PQ5

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Use

Tags

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQ5

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Use

Tags

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-U6a

Plasmid

#107593

Purpose

An entry vector with U6a promoter driving guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

U6a promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ211_WT_hi

Plasmid

#128850

Purpose

High copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK298

Plasmid

#59567

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4-FKBP12DD N-term C (w385-10)

Plasmid

#17416

Purpose

Gateway entry vector for Degron, N-term, frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2+E-Luc

Plasmid

#170873

Purpose

Phytobrick - E-Luc Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Mutation

c.375C>T to remove BbsI site

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP X2 DEST (w270-1)

Plasmid

#17474

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIN X2 DEST (w310-1)

Plasmid

#17399

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
TwinStrep-SUMO-Pnf

Plasmid

#198321

Purpose

For affinity purification of Pnf payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pnf

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_Δpvc10

Plasmid

#198448

Purpose

Produces PVCs containing truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_Δpvc10

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1251 - 1300 of 4271 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests